-
Northern Blotting
Northern BlottingAdapted from Molecular Cloning (Maniatis), Current Protocols in
2024-11-05 105 -
Top Ten Ways to Ensure Valid RNAi Data
１.Confirm results with a second or third siRNA to the same target. One of the
2024-11-05 83 -
Northern protocol(RULES FOR RNA WORK)
1. Wear gloves at all time including filling pipet tip in racks, filling
2024-11-05 69 -
How to prepare Molecular Biology grade glycogen
How to prepare Molecular Biology grade glycogenAuthor: Roberto SalviSource: Cont
2024-11-05 77 -
Purification of poly(A)+ RNA from total RNA
Purification of poly(A)+ RNA from total RNA0. (Optional)Before using Oligotex-dT
2024-11-05 74 -
RNA interference：The short answer
One way of seeing what a gene does is to block its messenger RNA and note the ef
2024-11-05 86 -
RNAi——制备siRNAs的几种方法
越来越多的研究人员开始采用小分子干扰RNA（small interfering RNAs，siRNAs)来抑制特定的哺乳动物基因表达。siRNA是一种短片断双链
2024-11-05 100 -
Preparation of single-stranded probes from cloned cDNA
a) 2-5 µg of plasmid DNA containing the cDNA insert is linearized using an appro
2024-11-05 88 -
IN SITU HYBRIDIZATION ON FROZEN SECTIONS
Remove slides from freezer, thaw for 5 min. at 55C. Fix 10 min. in 4% paraformal
2024-11-05 79 -
如何确定RNA质量的经验谈！
1） 检测RNA溶液的吸光度 280、320、230、260nm下的吸光度分别代表了核酸、背景（溶液浑浊度）、盐浓度和蛋白等有机物的值。一般的，我们只看OD26
2024-11-05 116 -
How to Maintain an RNase-free Lab
How to Maintain an RNase-free LabLet's admit it, everyone who has worked wit
2024-11-05 92 -
35S-RIBOPROBE SYNTHESIS FOR ISOTOPIC In Situ HYBRIDIZATION
Pipet 12.5µl 35S-UTP (1200 Ci/mmol) into 1.5ml microfuge tube. Final concentrati
2024-11-05 77 -
S1 analysis of yeast mRNA using oligonucleotide probes
S1 analysis of yeast mRNA using oligonucleotide probesSteve Hahn. last modified
2024-11-05 151 -
推荐：RNAi及其在植物遗传改良中的应用
RNAi即RNA干涉(或RNA干扰)是指双链RNA导入细胞后并被切割成21～23个核苷酸长度的短核苷酸双链，在其它作用因子的参与下能够特异地与其同源的mRNA结
2024-11-05 92 -
哺乳动物RNAi技术全面实验方法
WHENVIRUSESINFECTEUKARYOTICCELLS domly integrate into host genomes, double-stran
2024-11-05 85
