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DNA测序的基本原理及准备样品时的注意事项
一、测序的基本原理:测序的基本原理是Sanger末端终止法,在酶的作用下将原本是一条长达数百碱基的片断扩增成数百条片断,彼此间相差一个碱基。用电泳分离这些片断后
2025-05-13 6 -
染色体制备
一、 人外周血染色体制备1. 实验原理 人的外周血淋巴细胞培养方法是1960年由Moorhead 提出来的。正常情况下,人外周血小淋巴细胞都处在G1期(或G0期
2025-05-13 6 -
荧光素酶检测
IntroductionLuciferase can be used as a reporter gene to measure the activity of
2025-05-13 4 -
DNA Sequencing by Capillary Electrophoresis
IntroductionFor over 25 years, Applied Biosystems has been a pioneer in the fiel
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Summary: Illumina's Solexa Sequencing Technology
Here I present a brief overview of Solexa's sequencing-by-synthesis chemistr
2025-05-13 5 -
SOLiD Sequencing
Applied Biosystems has just launched their instrument, which supports their vers
2025-05-13 4 -
Sequencing Protocol
ABI SOLiD sequencing is a form of DNA sequencing. DNA sequencing encompasses bio
2025-05-13 4 -
ABI Solid Sequencing
ABI SOLiD sequencing is a form of DNA sequencing. DNA sequencing encompasses bio
2025-05-13 4 -
454 Sequencing
454 Life Sciences is a biotechnology company based in Branford, Connecticut spec
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测序技术
在分子生物学研究中,DNA的序列分析是进一步研究和改造目的基因的基础。目前用于测序的技术主要有Sanger等(1977)发明的双脱氧链末端终止法和Maxam和
2025-05-13 4 -
Protocol for Whole Genome Sequencing using Solexa Technology
The Solexa sequencing platform comprises all hardware, software, procedures and
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solexa sequencing
technology: illumina sequencing technologyThis breakthrough platform is based on
2025-05-13 5 -
SOLiD quality data
Q:We recently completed a ChIP-seq run on the ABI SOLiD. We received the followi
2025-05-13 4 -
Target enrichment technologies for ABI SOLiD
Q:my group is involved in a national project to develop a genomic platform for c
2025-05-13 4 -
Accuracy of SOLiD platform
Q:I've been getting mixed messages about the accuracy of the reads coming of
2025-05-13 4