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Purification and Functional Analysis of Human Properdin
Properdin is a member of the alternative pathway of complement. It is unique in
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Purification and Functional Characterization of Factor I
Factor I (FI) is a soluble, 88 kDa glycoprotein present in plasma at a concentra
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Purification of 20S Proteasomes
Proteasomes are large multicatalytic proteinases located in the nuclei and cytop
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Purification Of Anti Peptide Antibody
Protocol:All steps up to the dialysis at rt. Pour column in TBS (=0.15M NaCl, 20
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Purification of Antibodies and Preparation of Antibody Fragments
Antibodies may be purified from serum, ascitic fluid, and tissue culture superna
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Purification of Antibodies Using Affinity Chromatography
The effectiveness of affinity chromatography relies on the ability of a molecule
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Purification of Antibodies Using Ammonium Sulfate Fractionation or Gel Filtration
In this chapter, two commonly used techniques that are utilized in many immunogl
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Purification of Antibodies Using Ion-Exchange Chromatography
Ion-exchange chromatography is a rapid and inexpensive procedure employed to pur
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Purification of Antibodies Using Protein A-Sepharose and FPLC
Protein A chromatography is a type of affinity chromatography that relies on the
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Purification of Antibody Light Chains by Metal Affinity and Protein L Chromatography
Immobilized metal affinity chromatography (IMAC), introduced in 1975 (1 ), relie
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Purification of Antimicrobial Peptides from Human Skin
Human skin is a rich source of human antimicrobial peptides. Its cellular source
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Purification of C1q Receptors and Functional Analysis
The recognition subunit of C1, C1q, has emerged as an important player in variou
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Purification of Capsular Polysaccharide
Meningococcus is an aerobic, fastidious, Gram-negative diplococcus that is found
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Purification of Chemokines from Natural Sources
Chemokines (e.g., IL-8) were originally identified as chemotactic proteins obtai
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Purification of Complement Components, Regulators, and Receptors by Classical Methods
The aim of this chapter is to describe methods for purification of the individua
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