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Gene Transfer for Generation of Tumor and Leukemia Vaccines
Tumor vaccine strategies aim to compensate for the reduced immunogenicity of tum
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Gene Transfer of MHC-Restricted Receptors
Adoptive therapy with allogeneic or tumor-specific T-cells has shown substantial
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Gene Transfer to Articular Chondrocytes with Recombinant Adenovirus
Articular cartilage forms the load-bearing surfaces of diarthrodial joints. Its
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General Fusion Protocol
General Fusion ProtocolMaterials: P3X63Ag8.653 murine myeloma or YB2/0 (maintai
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Generating NK Cell Receptor-Fc Chimera Proteins from 293T Cells and Considerations of Appropriate Gl
The use of recombinant receptors as a scientific tool has become widespread in m
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Generation and Characterization of a Dual Variable Domain Immunoglobulin (DVD-Ig) Molecule
A dual-variable domain-immunoglobulin (DVD-Ig) is an engineered protein that com
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Generation and Culture of Mouse Embryonic Fibroblasts
The innate immune system is evolutionarily conserved and shared by a wide spectr
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Generation and Isolation of Target-Specific Single-Domain Antibodies from Shark Immune Repertoires
The drive to exploit novel targets and biological pathways has lead to the expan
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Generation and Selection of Rabbit Antibody Libraries by Phage Display
The rabbit antibody repertoire is an exceptional source for both polyclonal anti
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Generation and Testing of Immunoaffinity Columns
Immunoaffinity purification is a highly specific, reversible technique that has
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Generation of a Stable Cell Line for Constitutive miRNA Expression
miRNAs have in recent years emerged as novel players in virus–host interactions.
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Generation of a Tissue-Engineered Thymic Organoid
The thymic microenvironment provides essential support for the generation of a f
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Generation of Antibodies to Cell Surface Markers on Mature Natural Killer Cells
A limitation in the ability to analyze the function of the natural killer cell L
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Generation of Antigen-Specific, LacZ-Inducible T-Cell Hybrids
The recognition of a peptide-bound major histocompatibility complex (MHC) molecu
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Generation of Autologous Peptide- and Protein-Pulsed Dendritic Cells for Patient-Specific Immunother
This chapter presents a detailed protocol for the generation of mature, monocyte
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