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Phenolic Acids and Flavonoids: Occurrence and Analytical Methods
Phenolics are structurally assorted and are generally part of a complex mixture
2025-05-13 42 -
Live Cell Imaging of Chemotactic Dendritic Cell Migration in Explanted Mouse Ear Preparations
Leukocyte migration through the interstitial space is crucial for the maintenanc
2025-05-13 35 -
Iodogen碘化法
1.原理 用Iodogen为氧化剂,对蛋白质和多肽抗原进行碘化标记,把125I直接引进分子中的酪氨酸残基上。标记过程中被标记样品不与Iodogen混合,标记后取
2025-05-13 76 -
水解酪蛋白(MH)琼脂培养基如何配制?
1.营养肉汤培养基 牛肉膏 0.3克 蛋白胨 1.0克 NaCl 0.5克 水 100毫升 pH 7.0~7.2 在烧杯中加水,称取牛肉膏、蛋白胨
2025-05-13 44 -
聚丙烯酰胺凝胶电泳法
1.仪器装置 通常由稳流电泳仪和圆盘或平板电泳槽组成。其电泳室有上、下两槽,每个槽中都有固定的铂电极,铂电极经隔离电线接于电泳仪稳流挡上。2.试剂 (1)溶液A
2025-05-13 54 -
Working with RNA
Working with RNA is not a special discipline in molecular biology. However, RNA
2025-05-13 34 -
琼脂糖凝胶电泳法
1.仪器装置 电泳室及直流电源同纸电泳。 2.试剂 (1) 醋酸-锂盐缓冲液(pH3.0) 取冰醋酸50ml,加水800ml混合后,用氢氧化锂调节pH至3.0,
2025-05-13 44 -
纸电泳法
1.仪器装置 包括电泳室及直流电源两部分。 常用的水平式电泳室装置如图,包括两个电泳槽A和一个可以密封的玻璃(或相应材料)盖B;两侧的电泳槽均用有机玻璃(或相应
2025-05-13 37 -
柠檬脯
这是果脯蜜饯类食品,原料是柠檬盐胚。加工技术如下: 1.原料处理:咸柠檬脱盐,经浸水处理,采用流动水脱盐和经常换水脱盐时间大大缩短,否则用静水浸泡所需时间较长,
2025-05-13 34 -
Construction of Lambda Libraries from Large PFGE Fragments
Pulsed-field gel electrophoresis (PFGE) has the capacity to fractionate large fr
2025-05-13 37 -
Kinetic Measurements for Enzyme Immobilization
Enzyme kinetics is the study of the chemical reactions that are catalyzed by enz
2025-05-13 33 -
Vacuum-Microwave Combination for Processing Plant Tissues for Electron Microscopy
Preparation of botanical specimens for electron microscopy (EM) is hampered by s
2025-05-13 44 -
基因表达适应环境的变化
生物只有适应环境才能生存。当周围的营养、温度、湿度、酸度等条件变化时,生物体就要改变自身基因表达状况,以调整体内执行相应功能蛋白质的种类和数量,从而改变自身的代
2025-05-13 52 -
Lectin affinity electrophoresis
Lectin affinity electrophoresis is a powerful technique to investigate the inter
2025-05-13 42 -
柠檬李
新鲜李果一般风味欠佳,鲜食优良品种除外,大多数品种由于不耐贮藏而把它们加工成半成品盐胚,再加工成不同风味的凉果。为什么会有柠檬李制品?这是由于李胚本身除了咸、酸
2025-05-13 37