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Extending the Scope of Site-Specific Cysteine Bioconjugation by Appending a Prelabeled Cysteine Tag
Incorporating synthetic probes site-specifically into proteins is of central int
2025-04-12 10 -
Site-Directed Mutagenesis Using Double-Stranded Plasmid DNA Templates
In vitro site-directed mutagenesis is an invaluable technique for studying prote
2025-04-12 5 -
In Vitro Selection Procedures for Identifying DNA and RNA Aptamers Targeted to Nucleic Acids and Pro
In vitro selection or systematic evolution of ligands by exponential enrichment
2025-04-11 8 -
Isolation of Rat Hepatocytes
In vitro models, based on liver cells or tissues, are indispensable in the early
2025-04-11 9 -
In Vitro UDP-Sugar:Undecaprenyl-Phosphate Sugar-1-Phosphate Transferase Assay and Product Detection
In vitro assays are invaluable for the biochemical characterization of UDP-sugar
2025-04-11 5 -
In vitro Sphingomyelinase Assay
In vitro Sphingomyelinase Assay Contributor: Suprya JayadevDate: May 25, 1993Rea
2025-04-11 6 -
Generation of gene deletions and gene replacements in Escherichia coli O157:H7 using a temperature s
In this work we describe protocols for the generation of gene deletions and gene
2025-04-11 4 -
The method of contact angle measurements and estimation of work of adhesion in bioleaching of metals
In this paper, we present our method for the measurement of contact angles on th
2025-04-11 8 -
Preparation and Dynamic Patterning of Supported Lipid Membranes Mimicking Cell Membranes
In this chapter, we describe standardized protocols for the self-assembly of sup
2025-04-11 6 -
Heterologous Expression of GPCRs in Fission Yeast
In this chapter, we describe methods to heterologously express G protein-coupled
2025-04-11 6 -
Identification of Efficient Cleavage Sites in Long-Target RNAs
In this chapter, we describe a procedure for identification of efficient hammerh
2025-04-11 8 -
Ubiquitination of GPCRs
In this chapter, we describe a method for detecting the ubiquitination status of
2025-04-11 4 -
MALDI-MS Data Analysis for Disease Biomarker Discovery
In this chapter, we address the issue of matrix-assisted laser desorption/ioniza
2025-04-11 4 -
Fluorescent DNAzyme Biosensors for Metal Ions Based on Catalytic Molecular Beacons
In this chapter, methods for designing metal ion sensors using fluorophore- and
2025-04-11 5 -
Why Measure Drug Levels
In this chapter, I will discuss briefly some traditional uses of measurements of
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