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  • An Enhanced Antigen-Retrieval Protocol for Immunohistochemical Staining of Formalin-Fixed, Paraffin-

    An Enhanced Antigen-Retrieval Protocol for Immunohistochemical Staining of Formalin-Fixed, Paraffin-

    Formalin is the most commonly used fixative for light microscopy because of its

    2025-04-11 5
  • Phage-Display Libraries

    Phage-Display Libraries

    Foreign proteins can be displayed on the surface of filamentous bacteriophage by

    2025-04-11 6
  • Nanopore Force Spectroscopy on DNA Duplexes

    Nanopore Force Spectroscopy on DNA Duplexes

    Force spectroscopy can be applied using nanopores to study charged molecules suc

    2025-04-11 4
  • Characterizing Proteins from 2-DE Gels by Internal Sequence Analysis of Peptide Fragments: Strategie

    Characterizing Proteins from 2-DE Gels by Internal Sequence Analysis of Peptide Fragments: Strategie

    For years, proteolytic digest and peptide purification were essential tools for

    2025-04-11 6
  • Electrophoresis of DNA in Nondenaturing Polyacrylamide Gels

    Electrophoresis of DNA in Nondenaturing Polyacrylamide Gels

    For the majority of purposes, such as restriction mapping and sizing of cloned f

    2025-04-11 5
  • Small-Scale Plasmid DNA Preparation

    Small-Scale Plasmid DNA Preparation

    For the initial characterization of a recombinant plasmid, it is necessary to de

    2025-04-11 8
  • Synthesis of DNA Using a New Two-Step Cycle

    Synthesis of DNA Using a New Two-Step Cycle

    For the first time a new, two-step method is described for synthesizing deoxyrib

    2025-04-11 4
  • Encapsulation of Cells in Agarose Beads

    Encapsulation of Cells in Agarose Beads

    For the analysis of large DNA fragments using pulsed-field gel electrophoresis (

    2025-04-11 5
  • From No Expression to High-Level Soluble Expression in Escherichia coli by Screening a Library of th

    From No Expression to High-Level Soluble Expression in Escherichia coli by Screening a Library of th

    For structural studies by x-ray crystallography and nuclear magnetic resonance i

    2025-04-11 7
  • Bioluminescent Imaging of MAPK Function with Intein-Mediated Reporter Gene Assay

    Bioluminescent Imaging of MAPK Function with Intein-Mediated Reporter Gene Assay

    For nondestructive analysis of chemical processes in living mammalian cells, her

    2025-04-11 4
  • Ribotyping in Clinical Microbiology

    Ribotyping in Clinical Microbiology

    For a considerable fraction of patients who acquire a bacterial infection during

    2025-04-11 7
  • Direct cDNA Sequencing Using Sequential Linear/Asymmetric Polymerase Chain Reaction

    Direct cDNA Sequencing Using Sequential Linear/Asymmetric Polymerase Chain Reaction

    Following the isolation of a clonal recombinant phage or plasmid after screening

    2025-04-11 5
  • Production of Fluorous-Based Microarrays with Uncharged Carbohydrates

    Production of Fluorous-Based Microarrays with Uncharged Carbohydrates

    Fluorous-based carbohydrate microarrays provide an alternative to traditional co

    2025-04-11 5
  • Fluorochrome Absorption and Emission

    Fluorochrome Absorption and Emission

    Fluorochrome Absorption and Emission SpectraHere we present the absorption and e

    2025-04-11 4
  • Oligonucleotide Phosphorofluoridates and Fluoridites

    Oligonucleotide Phosphorofluoridates and Fluoridites

    Fluoro derivatives of phosphorus are of great importance in the chemistry and bi

    2025-04-11 4