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An Enhanced Antigen-Retrieval Protocol for Immunohistochemical Staining of Formalin-Fixed, Paraffin-
Formalin is the most commonly used fixative for light microscopy because of its
2025-04-11 5 -
Phage-Display Libraries
Foreign proteins can be displayed on the surface of filamentous bacteriophage by
2025-04-11 6 -
Nanopore Force Spectroscopy on DNA Duplexes
Force spectroscopy can be applied using nanopores to study charged molecules suc
2025-04-11 4 -
Characterizing Proteins from 2-DE Gels by Internal Sequence Analysis of Peptide Fragments: Strategie
For years, proteolytic digest and peptide purification were essential tools for
2025-04-11 6 -
Electrophoresis of DNA in Nondenaturing Polyacrylamide Gels
For the majority of purposes, such as restriction mapping and sizing of cloned f
2025-04-11 5 -
Small-Scale Plasmid DNA Preparation
For the initial characterization of a recombinant plasmid, it is necessary to de
2025-04-11 8 -
Synthesis of DNA Using a New Two-Step Cycle
For the first time a new, two-step method is described for synthesizing deoxyrib
2025-04-11 4 -
Encapsulation of Cells in Agarose Beads
For the analysis of large DNA fragments using pulsed-field gel electrophoresis (
2025-04-11 5 -
From No Expression to High-Level Soluble Expression in Escherichia coli by Screening a Library of th
For structural studies by x-ray crystallography and nuclear magnetic resonance i
2025-04-11 7 -
Bioluminescent Imaging of MAPK Function with Intein-Mediated Reporter Gene Assay
For nondestructive analysis of chemical processes in living mammalian cells, her
2025-04-11 4 -
Ribotyping in Clinical Microbiology
For a considerable fraction of patients who acquire a bacterial infection during
2025-04-11 7 -
Direct cDNA Sequencing Using Sequential Linear/Asymmetric Polymerase Chain Reaction
Following the isolation of a clonal recombinant phage or plasmid after screening
2025-04-11 5 -
Production of Fluorous-Based Microarrays with Uncharged Carbohydrates
Fluorous-based carbohydrate microarrays provide an alternative to traditional co
2025-04-11 5 -
Fluorochrome Absorption and Emission
Fluorochrome Absorption and Emission SpectraHere we present the absorption and e
2025-04-11 4 -
Oligonucleotide Phosphorofluoridates and Fluoridites
Fluoro derivatives of phosphorus are of great importance in the chemistry and bi
2025-04-11 4