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monoclonal antibody production fusion
Fusion is an important procedure to produce monoclonal antibodies (MoAb). PROTOC
2025-04-11 8 -
Measurement of Nitric Oxide-Related Enzymes in the Brain by In Situ Hybridization
Functional genomic increasingly requires the cell-specific localization of gene
2025-04-11 9 -
Cryopreservation of Rat Hepatocytes in a Three-Dimensional Culture Configuration Using a Controlled-
From engineered tissues to transfected cell lines, the long term storage of livi
2025-04-11 6 -
Recent advances in freeze-fracture electron microscop: the replica immunolabeling technique
Freeze-fracture electron microscopy is a technique for examining the ultrastruct
2025-04-11 9 -
Lipoic Acid as an Antioxidant: The Role of Dihydrolipoamide Dehydrogenase
Free lipoic acid is present in biological systems at extremely low levels. The b
2025-04-11 6 -
Fragment-Based Drug Design
Fragment-based drug design (FBDD), which is comprised of both fragment screening
2025-04-11 7 -
An Enhanced Antigen-Retrieval Protocol for Immunohistochemical Staining of Formalin-Fixed, Paraffin-
Formalin is the most commonly used fixative for light microscopy because of its
2025-04-11 8 -
Phage-Display Libraries
Foreign proteins can be displayed on the surface of filamentous bacteriophage by
2025-04-11 8 -
Nanopore Force Spectroscopy on DNA Duplexes
Force spectroscopy can be applied using nanopores to study charged molecules suc
2025-04-11 7 -
Characterizing Proteins from 2-DE Gels by Internal Sequence Analysis of Peptide Fragments: Strategie
For years, proteolytic digest and peptide purification were essential tools for
2025-04-11 11 -
Electrophoresis of DNA in Nondenaturing Polyacrylamide Gels
For the majority of purposes, such as restriction mapping and sizing of cloned f
2025-04-11 6 -
Small-Scale Plasmid DNA Preparation
For the initial characterization of a recombinant plasmid, it is necessary to de
2025-04-11 10 -
Synthesis of DNA Using a New Two-Step Cycle
For the first time a new, two-step method is described for synthesizing deoxyrib
2025-04-11 6 -
Encapsulation of Cells in Agarose Beads
For the analysis of large DNA fragments using pulsed-field gel electrophoresis (
2025-04-11 7 -
From No Expression to High-Level Soluble Expression in Escherichia coli by Screening a Library of th
For structural studies by x-ray crystallography and nuclear magnetic resonance i
2025-04-11 12