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Base-Modified Oligonucleotides With Increased Duplex Stability: Pyrazolo[3,4-d] Pyrimidines Replacin
Oligonucleotides incorporating 8-aza-7-dazapurines (pyrazolo[3,4-d]pyrimidines)
2025-04-14 10 -
Hybridization of Fluorescein-Labeled Oligonucleotide Probes and Enhanced Chemiluminescence Detection
Oligonucleotide synthesizers are now commonplace with the result that oligonucle
2025-04-14 10 -
Synthesis and Purification of Phosphorodithioate DNA
Oligonucleotide analogs bearing modified phosphodiester linkages have been the f
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Production of DNA Hybridization Probes with Digoxigenin-Modified Nucleotides by Random Hexanucleotid
Oligolabeling using random hexanucleotide primers (1 ) is an effective method fo
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Manual Oligonucleotide Synthesis Using the Phosphoramidite Method
Oligodeoxyribonucleotides may be synthesized on a solid support, which allows fo
2025-04-14 10 -
Multiplex Polymerase Chain Reaction
Often, studies require the polymerase chain reaction (PCR) amplification of seve
2025-04-14 12 -
DNA Profiling: Theory and Practice
Of the vast range of techniques in use in modern molecular biology, DNA protilin
2025-04-14 14 -
Cyclic Guanosine 5-Monophosphate Binding to Regulatory GAF Domains of Photoreceptor Phosphodiesteras
Of the 11 families of mammalian cyclic nucleotide phosphodiesterases (PDEs), 5 c
2025-04-14 14 -
Analysis of cGMP Signaling in Adipocytes
Obesity has reached pandemic dimensions with more than half a billion adults aff
2025-04-14 11 -
Synthesis of O-Glycopeptides and Construction of Glycopeptide Microarrays
O -Glycosylation of proteins is an important modification which affects biologic
2025-04-14 12 -
Study of GPCRProtein Interactions Using Gel Overlay Assays and Glutathione-S-Transferase-Fusion Prot
Numerous recent studies have suggested that the predicted cytosolic domains of G
2025-04-14 14 -
Quantification of Endogenous Retinoids
Numerous physiological processes require retinoids, including development, nervo
2025-04-14 13 -
Sequential Formation of Regioselective Disulfide Bonds in Synthetic Peptides with Multiple Disulfide
Numerous methods have been developed for the formation of disulfide bonds in rec
2025-04-14 14 -
Measurement of eNOS and iNOS mRNA Expression Using Reverse Transcription Polymerase Chain Reaction
Numerous cell populations are capable of producing nitric oxide (NO) from L -arg
2025-04-14 10 -
Photobiotin-Labeled DNA and RNA Hybridization Probes
Nucleic acid hybridization, the formation of a duplex between two complementary
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