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An Improved Method to Isolate Mitochondrial RNA from Green Plant Tissue
In plant cells, mitochondrial RNA (mtRNA) constitutes about only 1% of the total
2025-04-11 19 -
A Modified Coupled Enzyme Method for O-linked GlcNAc Transferase Activity Assay
In order to determine the activity of O-linked GlcNAc transferase (OGT), a modif
2025-04-11 17 -
Antibody cleanup--清除非特异抗体
In order to decrease the amount of nonspecific staining, it is often necessary t
2025-04-11 30 -
Use of Sulfolobus solfataricus PCNA Subunit Proteins to Direct the Assembly of Multimeric Enzyme Com
In nature, enzymes often form multienzyme complexes to enhance their catalytic e
2025-04-11 27 -
Detection and Characterization of Transcription Termination
In most eukaryotes, the generation of the 3′ end and transcription termination a
2025-04-11 17 -
Bilirubin and Its Carbohydrate Conjugates
In mammals, the open tetrapyrrole bilirubin (structure 2, Fig. 1 ) is the princi
2025-04-11 24 -
Detection of Aldehydic DNA Lesions Using Aldehyde Reactive Probe
In living cells, reactive oxygen species (ROS) such as superoxide anion, hydroge
2025-04-11 25 -
Zebrafish Spotted-Microarray for Genome-Wide Expression Profiling Experiments: Data Acquisition and
In expression microarray experiments, post-hybridization procedures involving da
2025-04-11 20 -
Studying Endoplasmic Reticulum Function In Vitro Using siRNA
In eukaryotic cells, N-glycosylation is typically the most common protein modifi
2025-04-11 22 -
Chromatin Endogenous Cleavage and Psoralen Crosslinking Assays to Analyze rRNA Gene Chromatin In Viv
In eukaryotes, multiple copies of ribosomal RNA (rRNA) genes co-exist in two dif
2025-04-11 19 -
Correlative Light and Electron Microscopy Using Immunolabeled Resin Sections
In correlative microscopy, light microscopy provides the overview and orientatio
2025-04-11 23 -
Identification of New Interacting Partners for Atypical Rho GTPases: A SILAC-Based Approach
In contrast to typical Rho GTPases the regulation of atypical Rho GTPases, such
2025-04-11 20 -
Detailed Structural Analysis of N-Glycans Released From Glycoproteins in SDS-PAGE Gel Bands Using HP
In contrast to the linear sequences of protein and DNA, oligosaccharides are bra
2025-04-11 19 -
Determination of Proteins, Phosphatidylethanolamine, and Phosphatidylserine in Lipid-Rich Materials
In classical, ninhydrin-based amino acid analysis (1 ,2 ), the ion-exchange matr
2025-04-11 26 -
4-Hydroxy-2-Nonenal (4-HNE) Staining by Anti-HNE Antibody
In almost all aerobic cells, the oxygen metabolism generates reactive oxygen spe
2025-04-11 20
