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  • Construction of Synthetic Genes by Polymerase Chain Reaction

    Construction of Synthetic Genes by Polymerase Chain Reaction

    Although the polymerase chain reaction (PCR) (1 ,2 ) is invaluable for the cloni

    2025-04-09 31
  • 2-D Protein Extracts from Drosophila melanogaster

    2-D Protein Extracts from Drosophila melanogaster

    Although the majority of proteins expressed in organisms with relatively low pro

    2025-04-09 27
  • Reverse Phase Protein Microarrays for Theranostics and Patient-Tailored Therapy

    Reverse Phase Protein Microarrays for Theranostics and Patient-Tailored Therapy

    Although the genome provides information about the somatic genetic changes exist

    2025-04-09 23
  • Immunoscreening of gt11 Expression Libraries Using an Avidin-Biotin Detection System

    Immunoscreening of gt11 Expression Libraries Using an Avidin-Biotin Detection System

    Although the avidin-biotin detection system has been used for immunochemical sta

    2025-04-09 27
  • Absorption Control in Immunohistochemistry Using Phospho-Peptides Immobilized on Magnetic Beads

    Absorption Control in Immunohistochemistry Using Phospho-Peptides Immobilized on Magnetic Beads

    Although phospho-specific primary antibodies used in immunohistochemistry (IHC)

    2025-04-09 30
  • Expression of Eukaryotic Cytochromes P450 in E. coli

    Expression of Eukaryotic Cytochromes P450 in E. coli

    Although much has been learned from the study of relatively abundant cytochromes

    2025-04-09 34
  • Micellar Polymer Encapsulation of Enzymes

    Micellar Polymer Encapsulation of Enzymes

    Although enzymes are highly efficient and selective catalysts, there have been p

    2025-04-09 27
  • Simultaneous Analysis of Intracellular pH and Ca2+ from Cell Populations

    Simultaneous Analysis of Intracellular pH and Ca2+ from Cell Populations

    Although changes in both pHin and [Ca2+ ]i have been observed in response to a v

    2025-04-09 21
  • In Vitro Assays to Analyze Translocation of the Model Secretory Preprotein Alkaline Phosphatase

    In Vitro Assays to Analyze Translocation of the Model Secretory Preprotein Alkaline Phosphatase

    Almost one-third of the proteins synthesized in the cytosol of cells ends up in

    2025-04-09 28
  • Selecting Allosteric Ribozymes

    Selecting Allosteric Ribozymes

    Allosteric ribozymes can be designed to respond to virtually any molecule of cho

    2025-04-09 33
  • (E)-Alkene Peptide Bond Isosteres by Cuprate Opening of Vinyl Aziridines

    (E)-Alkene Peptide Bond Isosteres by Cuprate Opening of Vinyl Aziridines

    Alkene isosteres are important nonhydrolyzable and rigidified analogs of peptide

    2025-04-09 38
  • Experimental assessment of the role of acetaldehyde in alcoholic cardiomyopathy

    Experimental assessment of the role of acetaldehyde in alcoholic cardiomyopathy

    Alcoholism is one of the major causes of non-ischemic heart damage. The myopathi

    2025-04-09 32
  • Intragastric Ethanol Infusion Model in Rodents

    Intragastric Ethanol Infusion Model in Rodents

    Alcohol-associated life-style disease, as exemplified by alcoholic liver disease

    2025-04-09 22
  • Assessment of Intracellular Mucin Content In Vivo

    Assessment of Intracellular Mucin Content In Vivo

    Airway mucus presents a first line of defense against inhaled materials. It also

    2025-04-09 26
  • Immunochemical Detection of a Fluorophore Derived from the Lipid Peroxidation Product 4-Hydroxy-2-No

    Immunochemical Detection of a Fluorophore Derived from the Lipid Peroxidation Product 4-Hydroxy-2-No

    Aging and the progression of certain degenerative diseases are accompanied by in

    2025-04-09 31