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The Geisler Method: Tracing Activity-Dependent cGMP Plasticity Changes upon Double Detection of mRNA
We recently demonstrated that an increase of guanosine 3′,5′-cyclic monophosphat
2025-03-31 13 -
Analysis of Rho GTPase Function in Axon Pathfinding Using Caenorhabditis elegans
We provide information and protocols for the analysis of Rho GTPase function in
2025-03-31 12 -
Comparative proteomics using 2-D gel electrophoresis and mass spectrometry as tools to dissect stimu
We propose two-dimensional gel electrophoresis (2-DE) and mass spectrometry to d
2025-03-31 14 -
Strategies for the Expression of SUMO-Modified Target Proteins in Escherichia coli
We previously described the establishment of a binary vector system that allows
2025-03-31 14 -
Microwave-Assisted Method for Fabrication of Carbohydrate Cluster Microarrays on 3-Dimensional Hydra
We present here a method for construction of carbohydrate cluster microarrays. T
2025-03-31 15 -
Deoxyribozyme-Based, Semisynthetic Access to Stable Peptidyl-tRNAs Exemplified by tRNAVal Carrying a
We present a protocol for the reliable synthesis of non-hydrolyzable 3′-peptidyl
2025-03-31 13 -
Computer Simulation of MAPK Signal Transduction
We predict that computational modeling platforms will soon become standard tools
2025-03-31 15 -
Modulation of human interferon- biosynthesis by antisense oligodeoxynucleotides
We investigated the inhibition of human interferon-γ (HuIFN-γ) production in cul
2025-03-31 16 -
Peptide substrate identification for yeast Hsp40 Ydj1 by screening the phage display library
We have identified a peptide substrate for molecular chaperone Hsp40 Ydj1 by uti
2025-03-31 16 -
A uniform procedure for the purification of CDK7/CycH/MAT1, CDK8/CycC and CDK9/CycT1
We have established a uniform procedure for the expression and purification of t
2025-03-31 19 -
A method for detecting functional activity related expression in gross brain regions, specific brain
We have developed a system to visualize functionally activated neurons and their
2025-03-31 15 -
Analyzing ligation mixtures using a PCR based method
We have developed a simple and effective method (Lig-PCR) for monitoring ligatio
2025-03-31 20 -
The Synthesis of Bicyclic Piperazinone and Related Derivatives
We have been examining synthetic approaches to conformationally restricted pepti
2025-03-31 14 -
Ligand Binding to Oligonucleotides
We have been developing a method for the study of small-molecule interactions wi
2025-03-31 20 -
Development of a new protocol for 2-day generation of mature dendritic cells from human monocytes
We developed a new 2-day protocol for the generation of dendritic cells (DCs) fr
2025-03-31 32
