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Fabrication of Polydimethylsiloxane Microfluidics Using SU-8 Molds
We detail the widely prevalent technique of polydimethylsiloxane (PDMS) molding
2025-03-31 16 -
Force and compliance measurements on living cells using atomic force microscopy (AFM)
We describe the use of atomic force microscopy (AFM) in studies of cell adhesion
2025-03-31 24 -
Biosensors Based on Carbon Nanotube-Network Field-Effect Transistors
We describe in detail the different steps involved in the construction of a carb
2025-03-31 15 -
Purification and Proteomic Analysis of a Nuclear-Insoluble Protein Fraction
We describe here a method for analyzing a rat liver nuclear-insoluble protein fr
2025-03-31 12 -
An improved method for constructing and selectively silanizing double-barreled, neutral liquid-carri
We describe an improved, efficient and reliable method for the vapour-phase sila
2025-03-31 11 -
An antibody-lectin sandwich assay for quantifying protein glycoforms
We describe an antibody-lectin sandwich assay for quantitation of glycoforms of
2025-03-31 20 -
Quantum Dot Hybrid Gel Blotting: A Technique for Identifying Quantum Dot-Protein/Protein-Protein Int
We describe an alternative to the molecular biology technique of polyacrylamide
2025-03-31 12 -
Systems for the Cell-Free Synthesis of Proteins
We describe a system for the cell-free expression of proteins based on extracts
2025-03-31 12 -
Semiquantitative RT-PCR analysis to assess the expression levels of multiple transcripts from the sa
We describe a semiquantitative RT-PCR protocol optimized in our laboratory to ex
2025-03-31 15 -
Quantitative Proteomics of Mouse Brain and Specific Protein-Interaction Studies Using Stable Isotope
We describe a new method for quantitative tissue proteomics using culture-derive
2025-03-31 11 -
Protein Processing Characterized by a Gel-Free Proteomics Approach
We describe a method for the specific isolation of representative N-terminal pep
2025-03-31 18 -
A Nonradioactive Assay for Nitric Oxide Synthase Activity in Tissue Extracts
We describe a fluorescence assay for nitric oxide synthase activity based on a n
2025-03-31 18 -
Quantification of Proteins and Metabolites by Mass Spectrometry Without Isotopic Labeling
We demonstrate the quantification capability and robustness of a new integrated
2025-03-31 14 -
In Vitro O-Antigen Ligase Assay
WaaL is a membrane enzyme that catalyzes the glycosidic bonding of a sugar at th
2025-03-31 17 -
Whole-Cell Patch-Clamp Recording of Voltage-Sensitive Ca2+ Channel Currents in Single Cells: Heterol
Voltage-sensitive calcium channels (VSCC) are vital to the normal physiology of
2025-03-31 16
