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  • Microscale Integrated Sperm Sorter

    Microscale Integrated Sperm Sorter

    This chapter describes the design and fabrication of a passively driven microflu

    2025-03-29 13
  • Linkers, Resins, and General Procedures for Solid-Phase Peptide Synthesis

    Linkers, Resins, and General Procedures for Solid-Phase Peptide Synthesis

    This chapter describes the basic protocols for solid-phase peptide synthesis usi

    2025-03-29 9
  • Adrenocorticotrophic Hormone and Related Peptides

    Adrenocorticotrophic Hormone and Related Peptides

    This chapter describes how adrenocorticotropin (ACTH) can be measured in biologi

    2025-03-29 14
  • Phage-Display Libraries of Murine and Human Antibody Fab Fragments

    Phage-Display Libraries of Murine and Human Antibody Fab Fragments

    This chapter describes efficient procedures for construction, expression, and sc

    2025-03-29 12
  • Processing Plant Tissues for Ultrastructural Study

    Processing Plant Tissues for Ultrastructural Study

    This chapter describes conventional chemical fixation methods and techniques for

    2025-03-29 16
  • Chemokine Receptor Antagonist Development

    Chemokine Receptor Antagonist Development

    This chapter describes assays that focus on the characterization of compounds id

    2025-03-29 11
  • Measurement of PTEN Activity in vivo by Imaging Phosphorylated Akt

    Measurement of PTEN Activity in vivo by Imaging Phosphorylated Akt

    This chapter describes an indirect approach to measure PTEN’s lipid phosphatase

    2025-03-29 14
  • Cryopreservation of Avian Spermatozoa

    Cryopreservation of Avian Spermatozoa

    This chapter describes a simple system for cryopreservation of avian spermatozoa

    2025-03-29 16
  • On-Resin Convergent Synthesis of a Glycopeptide from HIV gp120 Containing a High Mannose Type N-Link

    On-Resin Convergent Synthesis of a Glycopeptide from HIV gp120 Containing a High Mannose Type N-Link

    This chapter describes a rapid and efficient approach for the solid-phase synthe

    2025-03-29 13
  • Calibration of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Peptide Mass Fingerprintin

    Calibration of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Peptide Mass Fingerprintin

    This chapter describes a number of aspects important for calibration of matrix-a

    2025-03-29 11
  • Detection of mRNA in Whole Mounts of Mouse Embryos Using Digoxigenin Riboprobes

    Detection of mRNA in Whole Mounts of Mouse Embryos Using Digoxigenin Riboprobes

    This chapter describes a nonradioactive method for the localization of mRNA in w

    2025-03-29 15
  • Preparation of Oriented, Fully Hydrated Lipid Samples for Structure Determination Using X-Ray Scatte

    Preparation of Oriented, Fully Hydrated Lipid Samples for Structure Determination Using X-Ray Scatte

    This chapter describes a method of sample preparation called “the rock and roll

    2025-03-29 14
  • Production of Pseudotyped Retrovirus and the Generation of Proviral Transgenic Zebrafish

    Production of Pseudotyped Retrovirus and the Generation of Proviral Transgenic Zebrafish

    This chapter describes a method for generation of the high-titer pseudotyped Mol

    2025-03-29 9
  • Protein Fingerprinting of Staphylococcus aureus by Capillary Electrophoresis with On-Capillary Deriv

    Protein Fingerprinting of Staphylococcus aureus by Capillary Electrophoresis with On-Capillary Deriv

    This chapter describes a complete procedure for obtaining protein fingerprints o

    2025-03-29 11
  • Troubleshooting

    Troubleshooting

    This chapter covers the possible solutions to problems that may be encountered i

    2025-03-29 13