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  • Nitric Oxide Releasing Nanoparticle Synthesis and Characterization

    Nitric Oxide Releasing Nanoparticle Synthesis and Characterization

    While the potential applications of nitric oxide for both understanding human ph

    2025-03-31 25
  • Enzyme Stabilization and Immobilization by Sol-Gel Entrapment

    Enzyme Stabilization and Immobilization by Sol-Gel Entrapment

    While biocatalysts show tremendous potential for the industrial production of fi

    2025-03-31 27
  • Luminometric Measurement of Malate and Glucose-6-Phosphate in Mammalian Tissue

    Luminometric Measurement of Malate and Glucose-6-Phosphate in Mammalian Tissue

    When using cells of mammmalian origin, the amount of biological material availab

    2025-03-31 21
  • Follow-Up of Natural Product Isolation

    Follow-Up of Natural Product Isolation

    What do we mean by “follow-up”? Let us assume that the natural product just isol

    2025-03-31 32
  • Assessment of Cyclooxygenase Protein Expression by Western Blotting

    Assessment of Cyclooxygenase Protein Expression by Western Blotting

    Western blotting provides an effective, sensitive, and specific method for the i

    2025-03-31 26
  • Coupling Peptides To Resin For Affinity Purification

    Coupling Peptides To Resin For Affinity Purification

    We use Affigel-10, converting its functional group first to amino and then to io

    2025-03-31 28
  • The Geisler Method: Tracing Activity-Dependent cGMP Plasticity Changes upon Double Detection of mRNA

    The Geisler Method: Tracing Activity-Dependent cGMP Plasticity Changes upon Double Detection of mRNA

    We recently demonstrated that an increase of guanosine 3′,5′-cyclic monophosphat

    2025-03-31 27
  • Analysis of Rho GTPase Function in Axon Pathfinding Using Caenorhabditis elegans

    Analysis of Rho GTPase Function in Axon Pathfinding Using Caenorhabditis elegans

    We provide information and protocols for the analysis of Rho GTPase function in

    2025-03-31 23
  • Comparative proteomics using 2-D gel electrophoresis and mass spectrometry as tools to dissect stimu

    Comparative proteomics using 2-D gel electrophoresis and mass spectrometry as tools to dissect stimu

    We propose two-dimensional gel electrophoresis (2-DE) and mass spectrometry to d

    2025-03-31 24
  • Strategies for the Expression of SUMO-Modified Target Proteins in Escherichia coli

    Strategies for the Expression of SUMO-Modified Target Proteins in Escherichia coli

    We previously described the establishment of a binary vector system that allows

    2025-03-31 24
  • Microwave-Assisted Method for Fabrication of Carbohydrate Cluster Microarrays on 3-Dimensional Hydra

    Microwave-Assisted Method for Fabrication of Carbohydrate Cluster Microarrays on 3-Dimensional Hydra

    We present here a method for construction of carbohydrate cluster microarrays. T

    2025-03-31 30
  • Deoxyribozyme-Based, Semisynthetic Access to Stable Peptidyl-tRNAs Exemplified by tRNAVal Carrying a

    Deoxyribozyme-Based, Semisynthetic Access to Stable Peptidyl-tRNAs Exemplified by tRNAVal Carrying a

    We present a protocol for the reliable synthesis of non-hydrolyzable 3′-peptidyl

    2025-03-31 26
  • Computer Simulation of MAPK Signal Transduction

    Computer Simulation of MAPK Signal Transduction

    We predict that computational modeling platforms will soon become standard tools

    2025-03-31 30
  • Modulation of human interferon- biosynthesis by antisense oligodeoxynucleotides

    Modulation of human interferon- biosynthesis by antisense oligodeoxynucleotides

    We investigated the inhibition of human interferon-γ (HuIFN-γ) production in cul

    2025-03-31 31
  • Peptide substrate identification for yeast Hsp40 Ydj1 by screening the phage display library

    Peptide substrate identification for yeast Hsp40 Ydj1 by screening the phage display library

    We have identified a peptide substrate for molecular chaperone Hsp40 Ydj1 by uti

    2025-03-31 26