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  • A uniform procedure for the purification of CDK7/CycH/MAT1, CDK8/CycC and CDK9/CycT1

    A uniform procedure for the purification of CDK7/CycH/MAT1, CDK8/CycC and CDK9/CycT1

    We have established a uniform procedure for the expression and purification of t

    2025-03-31 30
  • A method for detecting functional activity related expression in gross brain regions, specific brain

    A method for detecting functional activity related expression in gross brain regions, specific brain

    We have developed a system to visualize functionally activated neurons and their

    2025-03-31 28
  • Analyzing ligation mixtures using a PCR based method

    Analyzing ligation mixtures using a PCR based method

    We have developed a simple and effective method (Lig-PCR) for monitoring ligatio

    2025-03-31 43
  • The Synthesis of Bicyclic Piperazinone and Related Derivatives

    The Synthesis of Bicyclic Piperazinone and Related Derivatives

    We have been examining synthetic approaches to conformationally restricted pepti

    2025-03-31 27
  • Ligand Binding to Oligonucleotides

    Ligand Binding to Oligonucleotides

    We have been developing a method for the study of small-molecule interactions wi

    2025-03-31 33
  • Development of a new protocol for 2-day generation of mature dendritic cells from human monocytes

    Development of a new protocol for 2-day generation of mature dendritic cells from human monocytes

    We developed a new 2-day protocol for the generation of dendritic cells (DCs) fr

    2025-03-31 42
  • Fabrication of Polydimethylsiloxane Microfluidics Using SU-8 Molds

    Fabrication of Polydimethylsiloxane Microfluidics Using SU-8 Molds

    We detail the widely prevalent technique of polydimethylsiloxane (PDMS) molding

    2025-03-31 28
  • Force and compliance measurements on living cells using atomic force microscopy (AFM)

    Force and compliance measurements on living cells using atomic force microscopy (AFM)

    We describe the use of atomic force microscopy (AFM) in studies of cell adhesion

    2025-03-31 38
  • Biosensors Based on Carbon Nanotube-Network Field-Effect Transistors

    Biosensors Based on Carbon Nanotube-Network Field-Effect Transistors

    We describe in detail the different steps involved in the construction of a carb

    2025-03-31 29
  • Purification and Proteomic Analysis of a Nuclear-Insoluble Protein Fraction

    Purification and Proteomic Analysis of a Nuclear-Insoluble Protein Fraction

    We describe here a method for analyzing a rat liver nuclear-insoluble protein fr

    2025-03-31 26
  • An improved method for constructing and selectively silanizing double-barreled, neutral liquid-carri

    An improved method for constructing and selectively silanizing double-barreled, neutral liquid-carri

    We describe an improved, efficient and reliable method for the vapour-phase sila

    2025-03-31 22
  • An antibody-lectin sandwich assay for quantifying protein glycoforms

    An antibody-lectin sandwich assay for quantifying protein glycoforms

    We describe an antibody-lectin sandwich assay for quantitation of glycoforms of

    2025-03-31 35
  • Quantum Dot Hybrid Gel Blotting: A Technique for Identifying Quantum Dot-Protein/Protein-Protein Int

    Quantum Dot Hybrid Gel Blotting: A Technique for Identifying Quantum Dot-Protein/Protein-Protein Int

    We describe an alternative to the molecular biology technique of polyacrylamide

    2025-03-31 24
  • Systems for the Cell-Free Synthesis of Proteins

    Systems for the Cell-Free Synthesis of Proteins

    We describe a system for the cell-free expression of proteins based on extracts

    2025-03-31 26
  • Semiquantitative RT-PCR analysis to assess the expression levels of multiple transcripts from the sa

    Semiquantitative RT-PCR analysis to assess the expression levels of multiple transcripts from the sa

    We describe a semiquantitative RT-PCR protocol optimized in our laboratory to ex

    2025-03-31 31