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A uniform procedure for the purification of CDK7/CycH/MAT1, CDK8/CycC and CDK9/CycT1
We have established a uniform procedure for the expression and purification of t
2025-03-31 30 -
A method for detecting functional activity related expression in gross brain regions, specific brain
We have developed a system to visualize functionally activated neurons and their
2025-03-31 28 -
Analyzing ligation mixtures using a PCR based method
We have developed a simple and effective method (Lig-PCR) for monitoring ligatio
2025-03-31 43 -
The Synthesis of Bicyclic Piperazinone and Related Derivatives
We have been examining synthetic approaches to conformationally restricted pepti
2025-03-31 27 -
Ligand Binding to Oligonucleotides
We have been developing a method for the study of small-molecule interactions wi
2025-03-31 33 -
Development of a new protocol for 2-day generation of mature dendritic cells from human monocytes
We developed a new 2-day protocol for the generation of dendritic cells (DCs) fr
2025-03-31 42 -
Fabrication of Polydimethylsiloxane Microfluidics Using SU-8 Molds
We detail the widely prevalent technique of polydimethylsiloxane (PDMS) molding
2025-03-31 28 -
Force and compliance measurements on living cells using atomic force microscopy (AFM)
We describe the use of atomic force microscopy (AFM) in studies of cell adhesion
2025-03-31 38 -
Biosensors Based on Carbon Nanotube-Network Field-Effect Transistors
We describe in detail the different steps involved in the construction of a carb
2025-03-31 29 -
Purification and Proteomic Analysis of a Nuclear-Insoluble Protein Fraction
We describe here a method for analyzing a rat liver nuclear-insoluble protein fr
2025-03-31 26 -
An improved method for constructing and selectively silanizing double-barreled, neutral liquid-carri
We describe an improved, efficient and reliable method for the vapour-phase sila
2025-03-31 22 -
An antibody-lectin sandwich assay for quantifying protein glycoforms
We describe an antibody-lectin sandwich assay for quantitation of glycoforms of
2025-03-31 35 -
Quantum Dot Hybrid Gel Blotting: A Technique for Identifying Quantum Dot-Protein/Protein-Protein Int
We describe an alternative to the molecular biology technique of polyacrylamide
2025-03-31 24 -
Systems for the Cell-Free Synthesis of Proteins
We describe a system for the cell-free expression of proteins based on extracts
2025-03-31 26 -
Semiquantitative RT-PCR analysis to assess the expression levels of multiple transcripts from the sa
We describe a semiquantitative RT-PCR protocol optimized in our laboratory to ex
2025-03-31 31