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  • Genome-Wide Analysis of Membrane Transport Using Yeast Knockout Arrays

    Genome-Wide Analysis of Membrane Transport Using Yeast Knockout Arrays

    The transport of membrane-bound proteins through post-Golgi compartments depends

    2025-03-29 10
  • Inserting and Manipulating DNA in a Nanopore with Optical Tweezers

    Inserting and Manipulating DNA in a Nanopore with Optical Tweezers

    The translocation of small molecules and polymers is an integral process for the

    2025-03-29 11
  • Gene Transfer and Expression in Tissue Culture Cells of Higher Eukaryotes

    Gene Transfer and Expression in Tissue Culture Cells of Higher Eukaryotes

    The transfer or “transfectron” of genetic material into tissue-culture cell line

    2025-03-29 10
  • Profiling RNA Polymerase II Using the Fast Chromatin Immunoprecipitation Method

    Profiling RNA Polymerase II Using the Fast Chromatin Immunoprecipitation Method

    The traditional method for determining the transcription rate of a gene, nuclear

    2025-03-29 10
  • Resin Technologies: Construction and Staining of Resin TMAs

    Resin Technologies: Construction and Staining of Resin TMAs

    The traditional formaldehyde-fixed paraffin-embedded tissue, and therefore the t

    2025-03-29 10
  • Affinity-Capture and Solid-Phase Sequencing of Biotinylated Polymerase Chain Reaction Products

    Affinity-Capture and Solid-Phase Sequencing of Biotinylated Polymerase Chain Reaction Products

    The time and labor required for generating nucleotide sequence information has b

    2025-03-29 16
  • Detection of ERK1/2 Activities Using Affinity Reagents

    Detection of ERK1/2 Activities Using Affinity Reagents

    The three major mammalian mitogen-activated protein kinase (MAPK) subfamilies in

    2025-03-29 10
  • Fluorometric and Colorimetric Assessment of Thiobarbituric Acid-Reactive Lipid Aldehydes in Biologic

    Fluorometric and Colorimetric Assessment of Thiobarbituric Acid-Reactive Lipid Aldehydes in Biologic

    The thiobarbituric acid (TBA) assay was developed to quantitatively determine li

    2025-03-29 13
  • On-Line Preconcentration and Separation of Antisense Oligonucleotides by ITP-CE in Dextran-Filled Ca

    On-Line Preconcentration and Separation of Antisense Oligonucleotides by ITP-CE in Dextran-Filled Ca

    The therapeutic use of “antisense oligonucleotides” is a new approach to rationa

    2025-03-29 17
  • The Isolation of Minicells

    The Isolation of Minicells

    The term minicell was introduced by Adler et al. (1 ) for the small spherical ce

    2025-03-29 14
  • Techniques for Determining the Metabolic Pathways of Eicosanoids and for Evaluating the Rate-Control

    Techniques for Determining the Metabolic Pathways of Eicosanoids and for Evaluating the Rate-Control

    The term eicosanoid is derived from the chemical name for arachidonic acid, eico

    2025-03-29 17
  • Scale-Up of Natural Products Isolation

    Scale-Up of Natural Products Isolation

    The term “scale-up” has been interpreted here as increasing the output of the pr

    2025-03-29 17
  • Gene Isolation by Exon Trapping

    Gene Isolation by Exon Trapping

    The technology of exon trapping, sometimes called exon amplification, strives to

    2025-03-29 18
  • Analysis of Yeast Artificial Chromosome Clones

    Analysis of Yeast Artificial Chromosome Clones

    The technology of cloning large fragments of DNA in yeast as yeast artificial ch

    2025-03-29 12
  • RNA Expression Analysis on Formalin-Fixed Paraffin-Embedded Tissues in TMA Format by RNA In Situ Hyb

    RNA Expression Analysis on Formalin-Fixed Paraffin-Embedded Tissues in TMA Format by RNA In Situ Hyb

    The technique of RNA in situ hybridization, i.e., the detection of specific mess

    2025-03-29 15