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  • Whole-Organism Screening: Plants

    Whole-Organism Screening: Plants

    The small plant Arabidopsis thaliana has been an indispensable tool for plant bi

    2025-03-29 16
  • Preparing 2-D Protein Extracts from Caenorhabditis elegans

    Preparing 2-D Protein Extracts from Caenorhabditis elegans

    The small nematode Caenorhabditis elegans (C. elegans , 1 mm in length) original

    2025-03-29 8
  • O-Glycoproteomics: Site-Specifi c O-Glycoprotein Analysis by CID/ETD Electrospray Ionization Tandem

    O-Glycoproteomics: Site-Specifi c O-Glycoprotein Analysis by CID/ETD Electrospray Ionization Tandem

    The sites of mucin-type O-glycosylation are difficult to predict, making structu

    2025-03-29 9
  • Single-Cell Gel Electrophoresis or Comet Assay of Intestinal Epithelial Cells Using Manual Scoring a

    Single-Cell Gel Electrophoresis or Comet Assay of Intestinal Epithelial Cells Using Manual Scoring a

    The single-cell gel electrophoresis or comet assay was initially reported in 198

    2025-03-29 12
  • An Asymmetric Synthesis Protocol to Prepare Topographically Constrained -Substituted Aromatic Amino

    An Asymmetric Synthesis Protocol to Prepare Topographically Constrained -Substituted Aromatic Amino

    The side chain groups of amino acid residues in polypeptide hormones, neurotrans

    2025-03-29 10
  • Analysis of Organelles by On-Line Two-Dimensional Liquid ChromatographyTandem Mass Spectrometry

    Analysis of Organelles by On-Line Two-Dimensional Liquid ChromatographyTandem Mass Spectrometry

    The sequencing of the genomes of many different species has greatly helped our u

    2025-03-29 11
  • Organizing Bioactive Compound Discovery in Target Families

    Organizing Bioactive Compound Discovery in Target Families

    The sequencing of genomes gave access to the complete set of building blocks for

    2025-03-29 11
  • Automated Protein Identification Using Microcolumn Liquid Chromatography-Tandem Mass Spectrometry

    Automated Protein Identification Using Microcolumn Liquid Chromatography-Tandem Mass Spectrometry

    The sequencing of complete genomes is providing an enormous resource for underst

    2025-03-29 17
  • Nucleic Acid Sequence Analysis Using DNAzymes

    Nucleic Acid Sequence Analysis Using DNAzymes

    The sequence specificity of the “10–23” RNA-cleaving DNA enzyme can be utilized

    2025-03-29 12
  • Messenger RNA Fractionation on Neutral Sucrose Gradients

    Messenger RNA Fractionation on Neutral Sucrose Gradients

    The separation of RNA on the basis of size by sucrose gradient fractionation is

    2025-03-29 12
  • Random Prime Labeling of DNA Probes with Fluorescein-11-dUTP

    Random Prime Labeling of DNA Probes with Fluorescein-11-dUTP

    The selection of an appropriate labeling reagent for a particular experiment, fo

    2025-03-29 9
  • Analysis of Nucleotide Sequence Variations by Solid-Phase Minisequencing

    Analysis of Nucleotide Sequence Variations by Solid-Phase Minisequencing

    The Sanger dideoxy-nucleotide sequencing method has been simplified by a number

    2025-03-29 9
  • S1 Mapping Using Single-Stranded DNA Probes

    S1 Mapping Using Single-Stranded DNA Probes

    The S1 nuclease is an endonuclease isolated from Aspergillus oryzae that digests

    2025-03-29 15
  • RNA Electrophoretic Mobility Shift Assay Using a Fluorescent DNA Sequencer

    RNA Electrophoretic Mobility Shift Assay Using a Fluorescent DNA Sequencer

    The RNA electrophoretic mobility shift assay is a simple and rapid method for vi

    2025-03-29 15
  • Posttranslational Lipid Modification of Rho Family Small GTPases

    Posttranslational Lipid Modification of Rho Family Small GTPases

    The Rho family comprises a major branch of the Ras superfamily of small GTPases.

    2025-03-29 16