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Immunohistochemical Localization of NOS Isoforms
Three distinct isoforms of nitric oxide synthases (NOS) have so far been identif
2025-03-31 20 -
Expression and purification strategies for the production of single-chain antibody and T-cell recept
This work describes protocols for the production of single-chain antibody and T-
2025-03-31 24 -
Method for introducing specific and unmarked mutations into the chromosome of Streptococcus pneumoni
This work describes a procedure for the generation of site-specific mutations in
2025-03-31 27 -
Dereplication and Partial Identification of Compounds
This review summarizes the advances in dereplication technology since 1998, its
2025-03-31 28 -
Large-scale immunofluorescence
This protocol describes our method for preparing cells for immunofluorescence, i
2025-03-31 24 -
High-copy cDNA amplification of minimal total RNA quantities for gene expression analyses
This protocol describes a PCR-based cDNA amplification technique of small total
2025-03-31 31 -
Isolation of LDL(Low Density Lipoprotein) From Human Plasma:
This procedure involves use of human plasma, a potentially dangerous source of b
2025-03-31 28 -
Techniques of EMG signal analysis: detection, processing, classification and applications (Correctio
This paper was originally published in Biological Procedures Online (BPO) on Mar
2025-03-31 28 -
Nutritional Value of d-Amino Acids, d-Peptides, and Amino Acid Derivatives in Mice
This paper describes a method for determining the nutritional value of d -amino
2025-03-31 31 -
Preparation and Separation of Intact Chromosomes of Vertebrates by One-Dimensional Pulsed-Field Gel
This novel way of preparing chromosomes for pulsed-field gel electrophoresis (PF
2025-03-31 20 -
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
This note is not intended as a full protocol for making monoclonal antibodies, b
2025-03-31 24 -
Protein Staining Procedures
This method was successful in our lab using prostate tissue and for our specific
2025-03-31 33 -
Subtilisin-Catalyzed Glycopeptide Condensation
This method describes the use of subtilisin-catalyzed peptide condensation to fo
2025-03-31 23 -
Novel Eicosanoids: Isoprostanes and Related Compounds
This chapter will summarize the biochemistry and biological actions of isoprosta
2025-03-29 17 -
Identification of SUMO-Interacting Proteins by Yeast Two-Hybrid Analysis
This chapter will discuss various adaptations of the yeast two-hybrid method for
2025-03-29 19