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Rapid (Ligase-Free) Subcloning of Polymerase Chain Reaction Products
The polymerase chain reaction (PCR) is a versatile, widely used method for the p
2025-03-29 20 -
Polymerase Chain Reaction: Basic Principles and Routine Practice
The polymerase chain reaction (PCR) is a primer-mediated enzymatic amplification
2025-03-29 14 -
Toward Effective PCR-Based Amplification of DNA on Microfabricated Chips
The polymerase chain reaction (PCR) has rapidly become the most valuable tool in
2025-03-29 14 -
HPLC of DNA and PCR Products
The polymerase chain reaction (PCR) has rapidly become a standard laboratory tec
2025-03-29 20 -
Production of Hybridization Probes by the PCR Utilizing Digoxigenin-Modified Nucleotides
The polymerase chain reaction (PCR) (1) is an efficient method for copying a fra
2025-03-29 18 -
Dynamic Monitoring of Cellular Remodeling Induced by the Transforming Growth Factor-1
The plasticity of differentiated adult cells could have a great therapeutic pote
2025-03-29 22 -
Purification of PDE6 Isozymes From Mammalian Retina
The photoreceptor phosphodiesterase (PDE6) is the central effector of visual tra
2025-03-29 17 -
Inositol 1,4,5-Trisphosphate Mass Assay
The phosphoinositide cascade plays a central role in the transduction of many ex
2025-03-29 14 -
Phosphatidylinositol(3,4,5)Trisphosphate (Ptdins(3,4,5)P3) Mass Measurement Using a Radioligand Disp
The phosphatidylinositol 3-kinases (PI 3-kinases), which play a pivotal role in
2025-03-29 23 -
Expression and Purification of Recombinant Proteins Using the pET System
The pET System is the most powerful system yet developed for the cloning and exp
2025-03-29 10 -
In Vivo Measurement of Tissue Oxygen Using Electron Paramagnetic Resonance Spectroscopy with Oxygen-
The partial pressure of oxygen (pO2 ) plays a determining role in the energy met
2025-03-29 19 -
Study of MAPK Signaling Using Knockout Mice
The p42 and p44 mitogen-activated protein kinases (MAPKs) (p42/p44 MAPK, Erk2 /E
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Post-electrophoretic identification of oxidized proteins
The oxidative modification of proteins has been shown to play a major role in a
2025-03-29 17 -
Immunohistochemical Detection of Protein Oxidation
The oxidative modification of proteins by reactive oxygen species (ROS) and othe
2025-03-29 26 -
Detection of Lipid Hydroperoxide-Derived Protein Modification with Polyclonal Antibodies
The oxidation of lipids can cause the formation of lipid hydroperoxide followed
2025-03-29 19
