-
Calibration of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Peptide Mass Fingerprintin
This chapter describes a number of aspects important for calibration of matrix-a
2025-03-29 20 -
Detection of mRNA in Whole Mounts of Mouse Embryos Using Digoxigenin Riboprobes
This chapter describes a nonradioactive method for the localization of mRNA in w
2025-03-29 23 -
Preparation of Oriented, Fully Hydrated Lipid Samples for Structure Determination Using X-Ray Scatte
This chapter describes a method of sample preparation called “the rock and roll
2025-03-29 24 -
Production of Pseudotyped Retrovirus and the Generation of Proviral Transgenic Zebrafish
This chapter describes a method for generation of the high-titer pseudotyped Mol
2025-03-29 22 -
Protein Fingerprinting of Staphylococcus aureus by Capillary Electrophoresis with On-Capillary Deriv
This chapter describes a complete procedure for obtaining protein fingerprints o
2025-03-29 20 -
Troubleshooting
This chapter covers the possible solutions to problems that may be encountered i
2025-03-29 26 -
Conventional Specimen Preparation Techniques for Transmission Electron Microscopy of Cultured Cells
This chapter covers the conventional methods and considerations for preparing cu
2025-03-29 22 -
Use of Natural or Selected Mutants and Variants for Epitope Mapping
This brief chapter is less of a protocol and more of a reminder that valuable in
2025-03-29 23 -
Principles of Liquid Chromatography
This article reviews the basic principles of high performance liquid chromatogra
2025-03-29 23 -
Gel Electrophoresis of RNA in Agarose and Polyacrylamide Under Nondenaturing Conditions
This article details two methods for separation and visualization of RNA under n
2025-03-29 25 -
In vitro transcription systems from cultured cells and fat-body tissue of the silkworm,Bombyx mori
This article describes the development of cell-free transcription systems from t
2025-03-29 21 -
Science through the internet: Researching, evaluating and citing websites
This article attempts to convey the joys and frustrations of skimming the Intern
2025-03-29 33 -
Cytofluorescence Techniques for the Visualization of Distinct Pools of Protein Thiols at the Single
Thiol groups of cysteine residues in protein have long been known as vulnerable
2025-03-29 22 -
GFP Reporter Screens for the Engineering of Amino Acid Degrading Enzymes from Libraries Expressed in
There is significant interest in engineering human amino acid degrading enzymes
2025-03-29 33 -
Special Problems with the Extraction of Plants
There is increasing scientific interest in the extraction and isolation of secon
2025-03-29 37
