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Lectin-Triggered Superoxide/H2O2 and Granule Enzyme Release from Cells
The modulatory potency of lectins on cellular activities deserves attention from
2025-03-29 55 -
Assaying Rho GTPase-Dependent Processes in Dictyostelium discoideum
The model organism D. discoideum is well suited to investigate basic questions o
2025-03-29 54 -
Ras Signaling Pathway for Analysis of Protein-Protein Interactions in Yeast and Mammalian Cells
The mitogen-activated protein kinase (MAPK) signaling pathway is initiated by di
2025-03-29 53 -
Preparation of Respiratory Chain Complexes from Saccharomyces cerevisiae Wild-Type and Mutant Mitoch
The mitochondrial oxidative phosphorylation involves five multimeric complexes i
2025-03-29 61 -
Fluorescence Measurement of Mitochondrial Membrane Potential Changes in Cultured Cells
The mitochondrial membrane potential is the dominant component of the proton-mot
2025-03-29 41 -
Phosphoinositidase C Activation Assay I: Cell Labeling, Stimulation, and Recovery of Cellular [3H]Ph
The minor inositol-containing membrane phospholipids, the phosphoinositides, pla
2025-03-29 46 -
Nanodroplet Chemical Microarrays and Label-Free Assays
The microarraying of chemicals or biomolecules on a glass surface allows for den
2025-03-29 63 -
Trapping Oxidative Folding Intermediates During Translocation to the Intermembrane Space of Mitochon
The MIA40 pathway is a novel import pathway in mitochondria specific for cystein
2025-03-29 38 -
Targeting mRNAs by Engineered Sequence-Specific RNase P Ribozymes
The methods of using engineered RNase P catalytic RNA (termed as M1GS RNA) for i
2025-03-29 40 -
Synthesis of DNA Mimics Representing HypNA-pPNA Hetero-Oligomers
The methods for the synthesis and purification of negatively charged peptide nuc
2025-03-29 42 -
PFGE Using Double-Inhomogeneous Fields or Orthogonal Field-Alternating Gel Electrophoresis (OFAGE)
The methods discussed in this chapter were the first pulsed-field gel electropho
2025-03-29 56 -
Proteolytic Labeling With 18O for Comparative Proteomics Studies: Preparation of 18O-Labeled Peptide
The method reported here uses proteolytic catalysis to introduce two 18O atoms i
2025-03-29 34 -
Use of Radiation Hybrid panels to map genetic loci
The mapping of genetic loci within organisms has been accelerated by the advent
2025-03-29 41 -
Characterization of Protein Glycosylation
The majority of proteins are posttranslationally modified, the most significant
2025-03-29 46 -
Chemical and Enzymatic Release of Glycans from Glycoproteins
The majority of proteins are posttranslationally modified, and the most signific
2025-03-29 37
