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  • Analysis of Carbohydrates by Mass Spectrometry

    Analysis of Carbohydrates by Mass Spectrometry

    The analysis method described in this chapter demonstrates the structural charac

    2025-03-27 19
  • High-Throughput Genotyping With Energy Transfer-Labeled Primers

    High-Throughput Genotyping With Energy Transfer-Labeled Primers

    The Amplifluor method for single-nucleotide polymorphisms (SNP) genotyping provi

    2025-03-27 19
  • The Amplification Refractory Mutation System

    The Amplification Refractory Mutation System

    The amplification refractory mutation system (ARMS) is a simple and rapid method

    2025-03-27 25
  • Detection of NOS Isoforms by Western-Blot Analysis

    Detection of NOS Isoforms by Western-Blot Analysis

    The amount of nitric oxide synthase (NOS) in tissues and cells can readily be de

    2025-03-27 22
  • The Analysis of Amino Acids Using Precolumn Derivatization, HPLC, and Electrochemical Detection

    The Analysis of Amino Acids Using Precolumn Derivatization, HPLC, and Electrochemical Detection

    The amino acids we have the most experience with are GABA, glutamate and arginin

    2025-03-27 17
  • Determination of Tyrosine and Tryptophan Metabolites in Body Ruids Using Electrochemical Detection

    Determination of Tyrosine and Tryptophan Metabolites in Body Ruids Using Electrochemical Detection

    The amino acids tyrosine and tryptophan are precursors for a number of important

    2025-03-27 20
  • Biophysical Approach to Determine the Subunit Stoichiometry of the Epithelial Sodium Channel Using t

    Biophysical Approach to Determine the Subunit Stoichiometry of the Epithelial Sodium Channel Using t

    The amiloride-sensitive epithelial Na+ channel (ENaC) is typically composed of t

    2025-03-27 19
  • Preparation, Restriction, and Hybridization Analysis of Mammalian Genomic DNA for Pulsed-Field Gel E

    Preparation, Restriction, and Hybridization Analysis of Mammalian Genomic DNA for Pulsed-Field Gel E

    The aim of this chapter is to provide a very practical set of instructions that

    2025-03-27 20
  • Purification and Enzymatic Sequencing of Polymerase Chain Reaction Products

    Purification and Enzymatic Sequencing of Polymerase Chain Reaction Products

    The advent of direct sequencing of polymerase chain reaction (PCR) products has

    2025-03-27 24
  • Generation of Epitope-Tagged GPCRs

    Generation of Epitope-Tagged GPCRs

    The addition of one or more epitope tags to G-protein-coupled receptors (GPCRs)

    2025-03-27 25
  • Acylcarnitines: Analysis in Plasma and Whole Blood Using Tandem Mass Spectrometry

    Acylcarnitines: Analysis in Plasma and Whole Blood Using Tandem Mass Spectrometry

    The acylcarnitine profile is a diagnostic test for inherited disorders of fatty

    2025-03-27 24
  • The Use of Translocating Fluorescent Biosensors for Real-Time Monitoring of GPCR-Mediated Signaling

    The Use of Translocating Fluorescent Biosensors for Real-Time Monitoring of GPCR-Mediated Signaling

    The ability to visualize the subcellular localization of proteins by labeling th

    2025-03-27 17
  • Systemic Delivery of Synthetic siRNAs

    Systemic Delivery of Synthetic siRNAs

    The ability to regulate endogenous gene expression by cleaving mRNA is important

    2025-03-27 22
  • Spectrophotometric Analysis of Nucleic Acids

    Spectrophotometric Analysis of Nucleic Acids

    The ability to quantify nucleic acids accurately and rapidly is a prerequisite f

    2025-03-27 16
  • Analysis of Urinary 8-oxo-7,8-dihydro-2-deoxyguanosine by Liquid ChromatographyTandem Mass Spectrome

    Analysis of Urinary 8-oxo-7,8-dihydro-2-deoxyguanosine by Liquid ChromatographyTandem Mass Spectrome

    The ability to non-invasively monitor DNA oxidation and its repair has significa

    2025-03-27 16