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Incorporation of Unnatural Non--Amino Acids into the N-Terminus of Proteins in a Cell-Free Translati
Unnatural amino acid mutagenesis allows us to introduce unnatural α-amino acids
2025-03-31 23 -
Chemical Cleavage (Maxam and Gilbert) Method for DNA Sequence Determination
Unlike the determination of the amino acid sequence of proteins and peptides, wh
2025-03-31 35 -
DOP-PCR Amplification of Whole Genomic DNA and Microchip-Based Capillary Electrophoresis
Universal or whole genome amplification by polymerase chain reaction (PCR) is a
2025-03-31 33 -
Chemogenomic Analysis of Safety Profiling Data
Understanding the safety of newly developed compounds is a key task in each earl
2025-03-31 26 -
Multiple Embryo Time-Lapse Imaging of Zebrafish Development
Understanding the dynamics of developmental and cellular processes requires docu
2025-03-31 29 -
Improved Cellular Uptake of Antisense Peptide Nucleic Acids by Conjugation to a Cell-Penetrating Pep
Unaided cellular uptake of RNA interference agents such as antisense oligonucleo
2025-03-31 32 -
Optimization of naked DNA delivery for interferon subtype immunotherapy in cytomegalovirus infection
Type I interferon (IFN) gene therapy modulates the immune response leading to in
2025-03-31 39 -
Bacterial Genome Mapping by Two-Dimensional Pulsed-Field Gel Electrophoresis (2D-PFGE)
Two-dimensional pulsed-field gel electrophoresis (2D-PFGE) is a powerful PFGE te
2025-03-31 35 -
Eukaryotic Cell Labeling and Preparation for 2-D
Two-dimensional polyacrylamide gel electrophoresis (2-DE) provides the ability t
2025-03-31 29 -
Computational Methods for Analysis of Two-Dimensional Gels
Two-dimensional gel electrophoresis (2D gels) is an essential quantitative prote
2025-03-31 22 -
Comparative Analysis of Cytoplasmic Membrane Proteomes of Escherichia coli Using 2D Blue Native/SDS-
Two-dimensional blue native (2D BN)/SDS-PAGE is the method of choice for the glo
2025-03-31 30 -
Cryopreservation of Mammalian Oocytes
Two methods for the cryopreservation of mammalian oocytes are described. One met
2025-03-31 29 -
Laser Tweezer Deformation of Giant Unilamellar Vesicles
Two methods are presented for deforming giant unilamellar vesicles with holograp
2025-03-31 35 -
Transverse Alternating-Field Electrophoresis
Transverse alternating-field electrophoresis (TAFE) refers to a pulsed-field sys
2025-03-31 35 -
New Fluorescent Substrates of Microbial Transglutaminase and Its Application to Peptide Tag-Directed
Transglutaminase (TGase) is an enzyme that catalyzes the post-translational cova
2025-03-31 41
