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Directed Mutagenesis of Antibody Variable Domains
The ability to mutate antibodies at the genetic level is a powerful tool in the
2025-03-27 28 -
Tobramycin Affinity Tag Purification of Spliceosomes
The ability to isolate native ribonucleoprotein (RNP) particles is of fundamenta
2025-03-27 22 -
Site-Directed Mutagenesis Using a Uracil-Containing Phagemid Template
The ability to introduce specific changes into almost any given DNA sequence has
2025-03-27 20 -
Improved method for generating microarray probes using submicrogram amounts of total RNA
The ability to identify disease-associated genes using microarray technology is
2025-03-27 16 -
Peptide Nucleic Acid-Mediated Recombination for Targeted Genomic Repair and Modification
The ability to directly manipulate the human genome to correct a disease-related
2025-03-27 22 -
Site-Directed Mutagenesis
The ability to create defined mutations and to generate precise fusions of prote
2025-03-27 21 -
Restriction Endonuclease Digestion of DNA
The ability to cleave DNA at specific sites is one of the cornerstones of today’
2025-03-27 15 -
Detection of Total Proteins on Western Blots of 2-D Polyacrylamide Gels
The ability of two-dimensional electrophoresis (2-DE), based on the original met
2025-03-27 18 -
Target-Site Selection for the 1023 DNAzyme
The 10–23 DNAzyme is capable of cleaving RNA with high sequence specificity at s
2025-03-27 24 -
An efficient ligation method in the making of an in vitro virus for in vitro protein evolution
The “in vitro virus” is a molecular construct to perform evolutionary protein en
2025-03-27 26 -
Application of a colorimetric assay to identify putative ribofuranosylaminobenzene 5-phosphate synth
Tetrahydromethanopterin (H4 MPT) is a tetrahydrofolate analog originally discove
2025-03-27 25 -
Analysis of Average Telomere Length in Cultured Human Cells
Telomeres play an important role in ensuring the integrity of the genome. Telome
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Introduction to Telomeres and Telomerase
Telomeres are ends of chromosomes that play an important part in the biology of
2025-03-27 20 -
Probing the Telomere Damage Response
Telomere dysfunctions, rendered through replicative attrition of telomeric DNA o
2025-03-27 21 -
Telomere Terminal G/C Strand Synthesis: Measuring Telomerase Action and C-Rich Fill-In
Telomerase is present in most human cancers, and proliferative stem cells includ
2025-03-27 22
