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Sequencing DNA Fragments Cloned into M13 and Phagemid Vectors
The dideoxy chain-termination method (1 ) involves enzymatic elongation of a oli
2025-03-27 42 -
Amino Acid Profiling for the Diagnosis of Inborn Errors of Metabolism
The diagnosis of inherited metabolic disorders of amino acid (AA) metabolism is
2025-03-27 45 -
Purification of Synthetic Oligonucleotides by Preparative Gel Electrophoresis
The development of the chemistry for solid-phase oligonucleotide synthesis has e
2025-03-27 37 -
Chiral Separation Principles: An Introduction
The development of methods for chiral separation on an analytical as well as on
2025-03-27 36 -
Generation of PDE4 Knockout Mice by Gene Targeting
The development of gene-targeting techniques has ushered in a new era in mouse g
2025-03-27 36 -
Automated Liquid-Liquid Extraction and Ion-Exchange Solid-Phase Extraction for Initial Purification
The development of combinatorial chemistry has made a profound impact on the pha
2025-03-27 50 -
In Vivo ChIP for the Analysis of Microdissected Tissue Samples
The development of chromatin immunoprecipitation assays (ChIP) as a tool to exam
2025-03-27 51 -
In Vivo Screening of Ligand-Dependent Hammerhead Ribozymes
The development of artificial switches of gene expression is of high importance
2025-03-27 39 -
Using the Flp-In T-Rex System to Regulate GPCR Expression
The development of a cell-based system that allows the integration of a gene of
2025-03-27 41 -
An Overview of CE in Clinical Analysis
The development and general applications of capillary electrophoresis (CE) in th
2025-03-27 57 -
On-Line SFE-SFC for the Analysis of Fat-Soluble Vitamins and Other Lipids from Water Matrices
The determination of trace organic compounds in aqueous samples usually involves
2025-03-27 40 -
Southern Blotting of Agarose Gels by Capillary Transfer
The detection of specific nucleic acid species following electrophoretic separat
2025-03-27 36 -
Double-Label Analysis
The detection of radiolabeled proteins is of fundamental importance in experimen
2025-03-27 47 -
Hydroxyl and 1-Hydroxyethyl Radical Detection by Spin Trapping and GC-MS
The detection of free radicals is often carried out by electron spin resonance (
2025-03-27 47 -
Small-Angle Neutron Scattering to Detect Rafts and Lipid Domains
The detection and characterization of lateral heterogeneities or domains in lipi
2025-03-27 45