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  • Sequencing DNA Fragments Cloned into M13 and Phagemid Vectors

    Sequencing DNA Fragments Cloned into M13 and Phagemid Vectors

    The dideoxy chain-termination method (1 ) involves enzymatic elongation of a oli

    2025-03-27 42
  • Amino Acid Profiling for the Diagnosis of Inborn Errors of Metabolism

    Amino Acid Profiling for the Diagnosis of Inborn Errors of Metabolism

    The diagnosis of inherited metabolic disorders of amino acid (AA) metabolism is

    2025-03-27 45
  • Purification of Synthetic Oligonucleotides by Preparative Gel Electrophoresis

    Purification of Synthetic Oligonucleotides by Preparative Gel Electrophoresis

    The development of the chemistry for solid-phase oligonucleotide synthesis has e

    2025-03-27 37
  • Chiral Separation Principles: An Introduction

    Chiral Separation Principles: An Introduction

    The development of methods for chiral separation on an analytical as well as on

    2025-03-27 36
  • Generation of PDE4 Knockout Mice by Gene Targeting

    Generation of PDE4 Knockout Mice by Gene Targeting

    The development of gene-targeting techniques has ushered in a new era in mouse g

    2025-03-27 36
  • Automated Liquid-Liquid Extraction and Ion-Exchange Solid-Phase Extraction for Initial Purification

    Automated Liquid-Liquid Extraction and Ion-Exchange Solid-Phase Extraction for Initial Purification

    The development of combinatorial chemistry has made a profound impact on the pha

    2025-03-27 50
  • In Vivo ChIP for the Analysis of Microdissected Tissue Samples

    In Vivo ChIP for the Analysis of Microdissected Tissue Samples

    The development of chromatin immunoprecipitation assays (ChIP) as a tool to exam

    2025-03-27 51
  • In Vivo Screening of Ligand-Dependent Hammerhead Ribozymes

    In Vivo Screening of Ligand-Dependent Hammerhead Ribozymes

    The development of artificial switches of gene expression is of high importance

    2025-03-27 39
  • Using the Flp-In T-Rex System to Regulate GPCR Expression

    Using the Flp-In T-Rex System to Regulate GPCR Expression

    The development of a cell-based system that allows the integration of a gene of

    2025-03-27 41
  • An Overview of CE in Clinical Analysis

    An Overview of CE in Clinical Analysis

    The development and general applications of capillary electrophoresis (CE) in th

    2025-03-27 57
  • On-Line SFE-SFC for the Analysis of Fat-Soluble Vitamins and Other Lipids from Water Matrices

    On-Line SFE-SFC for the Analysis of Fat-Soluble Vitamins and Other Lipids from Water Matrices

    The determination of trace organic compounds in aqueous samples usually involves

    2025-03-27 40
  • Southern Blotting of Agarose Gels by Capillary Transfer

    Southern Blotting of Agarose Gels by Capillary Transfer

    The detection of specific nucleic acid species following electrophoretic separat

    2025-03-27 36
  • Double-Label Analysis

    Double-Label Analysis

    The detection of radiolabeled proteins is of fundamental importance in experimen

    2025-03-27 47
  • Hydroxyl and 1-Hydroxyethyl Radical Detection by Spin Trapping and GC-MS

    Hydroxyl and 1-Hydroxyethyl Radical Detection by Spin Trapping and GC-MS

    The detection of free radicals is often carried out by electron spin resonance (

    2025-03-27 47
  • Small-Angle Neutron Scattering to Detect Rafts and Lipid Domains

    Small-Angle Neutron Scattering to Detect Rafts and Lipid Domains

    The detection and characterization of lateral heterogeneities or domains in lipi

    2025-03-27 45