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  • Screening Recombinant Libraries by Polymerase Chain Reaction

    Screening Recombinant Libraries by Polymerase Chain Reaction

    The complexity of the genome of a particular organism or the relative abundance

    2025-03-27 52
  • The Human Genome Project

    The Human Genome Project

    The completion of the Human Genome Project (HGP) was announced on 24 April 2003

    2025-03-27 41
  • A Novel Strategy for a Splice-Variant Selective Gene Ablation: The Example of the Versican V0/V2 Kno

    A Novel Strategy for a Splice-Variant Selective Gene Ablation: The Example of the Versican V0/V2 Kno

    The complete knockout of genes that give rise to alternative splice products can

    2025-03-27 53
  • Spectroscopy-Based Quantitative Fluorescence Resonance Energy Transfer Analysis

    Spectroscopy-Based Quantitative Fluorescence Resonance Energy Transfer Analysis

    The combination of green fluorescent protein mutants and fluorescence resonance

    2025-03-27 43
  • Analyzing PPAR/Ligand Interactions by Chemical Cross-Linking and High-Resolution Mass Spectrometry

    Analyzing PPAR/Ligand Interactions by Chemical Cross-Linking and High-Resolution Mass Spectrometry

    The combination of chemical cross-linking and high-resolution mass spectrometry

    2025-03-27 40
  • Plasmid DNA Isolation by the Cleared Lysate Method

    Plasmid DNA Isolation by the Cleared Lysate Method

    The cleared lysate method of plasmid isolation is commonly used to extract relat

    2025-03-27 42
  • Using Chromatin Immunoprecipitation to Map Cotranscriptional mRNA Processing in Saccharomyces cerevi

    Using Chromatin Immunoprecipitation to Map Cotranscriptional mRNA Processing in Saccharomyces cerevi

    The chromatin immunoprecipitation (ChIP) technique has been used to determine wh

    2025-03-27 49
  • Indirect Enantioseparation by HPLC Using Chiral Benzofurazan-Bearing Reagents

    Indirect Enantioseparation by HPLC Using Chiral Benzofurazan-Bearing Reagents

    The chiral resolutions by high-performance liquid chromatography (HPLC) are divi

    2025-03-27 38
  • Synthesis by high-efficiency liquid-phase (HELP) method of oligonucleotides conjugated with high-mol

    Synthesis by high-efficiency liquid-phase (HELP) method of oligonucleotides conjugated with high-mol

    The chemical modification of synthetic oligonucleotides has recently been invest

    2025-03-27 57
  • Detection of Unique or Low Copy Number DNA Sequences in Complex Genomes

    Detection of Unique or Low Copy Number DNA Sequences in Complex Genomes

    The characterization of specific DNA sequences by means of hybridization techniq

    2025-03-27 75
  • O-Glycoprotein Biosynthesis: Site Localization by Edman Degradation and Site Prediction Based on Ran

    O-Glycoprotein Biosynthesis: Site Localization by Edman Degradation and Site Prediction Based on Ran

    The characterization of mucin-type O-glycosylation is fraught with extreme diffi

    2025-03-27 51
  • Affinity Measurement Using Surface Plasmon Resonance

    Affinity Measurement Using Surface Plasmon Resonance

    The change in free energy for a reaction, ΔG, can be determined from the dissoci

    2025-03-27 40
  • Protocol for Ex Vivo Incubation of Drosophila Primary Post-embryonic Haemocytes for Real-Time Analys

    Protocol for Ex Vivo Incubation of Drosophila Primary Post-embryonic Haemocytes for Real-Time Analys

    The cellular branch of the Drosophila larval innate immune system consists of th

    2025-03-27 35
  • A method for analyzing the ubiquitination and degradation of aurora-A

    A method for analyzing the ubiquitination and degradation of aurora-A

    The cell cycle machinery consists of regulatory proteins that control the progre

    2025-03-27 45
  • Design and Preparation of Sequence-Specific RNase P Ribozymes

    Design and Preparation of Sequence-Specific RNase P Ribozymes

    The catalytic RNA subunit of Escherichia coli RNase P (M1 RNA) is a structure-sp

    2025-03-27 47