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Weak Affinity Chromatography
Since the inception of affinity chromatography 30 years ago (1 ), it has develop
2025-03-27 24 -
Inverse Polymerase Chain Reaction
Since the first report on cDNA cloning in 1972 (1 ), this technology has been de
2025-03-27 16 -
Development of a High-Throughput Capillary Electrophoresis Protocol for DNA Fragment Analysis
Since the first descriptions of electrophoresis in small diameter tubes in the 1
2025-03-27 21 -
Production of Panels of Monoclonal Antibodies by the Hybridoma Method
Since the first description by Kohler and Milstein (1 ), many variations on this
2025-03-27 19 -
Developing a Minimally Immunogenic Humanized Antibody by SDR Grafting
Since the advent of hybridoma technology a quarter century ago, a large number o
2025-03-27 14 -
Mapping of Signaling Pathways by Functional Interaction Proteomics
Signaling pathways transduce extracellular stimuli from the membrane to the nucl
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General Consideration on Sialic Acid Chemistry
Sialic acids, also known as neuraminic acids, are a family of negatively charged
2025-03-27 15 -
Shotgun phage display Selection for bacterial receptins or other exported proteins
Shotgun phage display cloning involves construction of libraries from randomly f
2025-03-27 19 -
Detecting Ordered Domain Formation (Lipid Rafts) in Model Membranes Using Tempo
Short-range fluorescence quenching has proven to be an effective method to detec
2025-03-27 15 -
Cryopreservation of Shoot Tips and Meristems
Shoot-tip meristem cryopreservation methodologies are reported for the complemen
2025-03-27 18 -
Oscillating Probe for Dual Detection of 5'PO4 and 5'OH DNA Breaks in Tissue Sections
Several types of DNA cuts are used as markers of apoptosis for detection of apop
2025-03-27 18 -
Nonradioactive Northern and Southern Analyses From Plant Samples
Several specific problems are encountered when nonradioactive detection methods
2025-03-27 14 -
Use of three-dimensional collagen gels to study mechanotransduction in t47d breast epithelial cells
Several pathological and disease conditions can alter the mechanical properties
2025-03-27 18 -
Dealing with different methods for Kluyveromyces lactis -galactosidase purification
Several micro-scale chromatography-based procedures for purification of the β-ga
2025-03-27 12 -
Formation and Characterization of Hepatocyte Spheroids
Several investigators have demonstrated that freshly harvested hepatocytes self-
2025-03-27 17
