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An efficient ligation method in the making of an in vitro virus for in vitro protein evolution
The “in vitro virus” is a molecular construct to perform evolutionary protein en
2025-03-27 50 -
Application of a colorimetric assay to identify putative ribofuranosylaminobenzene 5-phosphate synth
Tetrahydromethanopterin (H4 MPT) is a tetrahydrofolate analog originally discove
2025-03-27 44 -
Analysis of Average Telomere Length in Cultured Human Cells
Telomeres play an important role in ensuring the integrity of the genome. Telome
2025-03-27 56 -
Introduction to Telomeres and Telomerase
Telomeres are ends of chromosomes that play an important part in the biology of
2025-03-27 42 -
Probing the Telomere Damage Response
Telomere dysfunctions, rendered through replicative attrition of telomeric DNA o
2025-03-27 47 -
Telomere Terminal G/C Strand Synthesis: Measuring Telomerase Action and C-Rich Fill-In
Telomerase is present in most human cancers, and proliferative stem cells includ
2025-03-27 54 -
Probing Antibody-Antigen Interactions by Mass Spectrometry
Techniques that have been used for antigenic site mapping of linear epitopes in
2025-03-27 38 -
Practical Considerations for Analyzing Antigene RNAs (agRNAs): RNA Immunoprecipitation of Argonaute
Target validation for small RNAs in cells can be a confusing task wrought with p
2025-03-27 45 -
Tandem Mass Spectrometry in the Detection of Inborn Errors of Metabolism for Newborn Screening
Tandem mass spectrometry has been used for determinations of enzyme activities i
2025-03-27 49 -
Interpretation of Collision-Induced Fragmentation Tandem Mass Spectra of Posttranslationally Modifie
Tandem collision-induced dissociation (CID) mass spectrometry (MS) provides a se
2025-03-27 47 -
TAL Effector Nuclease (TALEN) Engineering
TALENs, fusion proteins of DNA binding domains of TAL (transcription activator-l
2025-03-27 54 -
Integration of Phosphodiesterase-Induced Degradation of Oligonucleotides with Capillary Polymer-Siev
Synthetic oligodeoxyribonucleotides (ODN) have been proposed as a class of poten
2025-03-27 57 -
Transcription In Vitro Using Bacteriophage RNA Polymerases
Synthesis of specific RNA sequences in vitro is simplified because of the availa
2025-03-27 36 -
Chemical and Enzymatic Approaches to Construct Modified RNAs
Synthesis of modified nucleotides and then incorporation into an RNA chain have
2025-03-27 50 -
Surfactant-Based Methods for Prevention of Protein Adsorption in Capillary Electrophoresis
Surfactants such as didodecyldimethyl ammonium bromide (DDAB) and 1,2-dilauroyl-
2025-03-27 54