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Single Enzyme Studies: A Historical Perspective
Single-molecule enzymology has a longer history than is often supposed, with the
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DNA-Templated Native Chemical Ligation of Functionalized Peptide Nucleic Acids: A Versatile Tool for
Single base-specific detection of DNA/RNA sequences is of importance in the diag
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Ligation of DNA with T4 DNA Ligase
Since they are involved in such important processes as DNA replication, DNA repa
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Yeast Artificial Chromosomes
Since their mtroductlon in 1987, yeast artificial chromosomes (commonly referred
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Separation of Enantiomers by Capillary Electrophoresis Using Cyclodextrins
Since the pioneering publication of first paper on application of capillary elec
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Using a Noncovalent Protection Strategy to Enhance Solid-Phase Synthesis
Since the introduction of solid-phase peptide synthesis by Merrifield (1 ) nearl
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Weak Affinity Chromatography
Since the inception of affinity chromatography 30 years ago (1 ), it has develop
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Inverse Polymerase Chain Reaction
Since the first report on cDNA cloning in 1972 (1 ), this technology has been de
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Development of a High-Throughput Capillary Electrophoresis Protocol for DNA Fragment Analysis
Since the first descriptions of electrophoresis in small diameter tubes in the 1
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Production of Panels of Monoclonal Antibodies by the Hybridoma Method
Since the first description by Kohler and Milstein (1 ), many variations on this
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Developing a Minimally Immunogenic Humanized Antibody by SDR Grafting
Since the advent of hybridoma technology a quarter century ago, a large number o
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Mapping of Signaling Pathways by Functional Interaction Proteomics
Signaling pathways transduce extracellular stimuli from the membrane to the nucl
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General Consideration on Sialic Acid Chemistry
Sialic acids, also known as neuraminic acids, are a family of negatively charged
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Shotgun phage display Selection for bacterial receptins or other exported proteins
Shotgun phage display cloning involves construction of libraries from randomly f
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Detecting Ordered Domain Formation (Lipid Rafts) in Model Membranes Using Tempo
Short-range fluorescence quenching has proven to be an effective method to detec
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