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  • Improved method for generating microarray probes using submicrogram amounts of total RNA

    Improved method for generating microarray probes using submicrogram amounts of total RNA

    The ability to identify disease-associated genes using microarray technology is

    2025-03-27 39
  • Peptide Nucleic Acid-Mediated Recombination for Targeted Genomic Repair and Modification

    Peptide Nucleic Acid-Mediated Recombination for Targeted Genomic Repair and Modification

    The ability to directly manipulate the human genome to correct a disease-related

    2025-03-27 57
  • Site-Directed Mutagenesis

    Site-Directed Mutagenesis

    The ability to create defined mutations and to generate precise fusions of prote

    2025-03-27 45
  • Restriction Endonuclease Digestion of DNA

    Restriction Endonuclease Digestion of DNA

    The ability to cleave DNA at specific sites is one of the cornerstones of today’

    2025-03-27 43
  • Detection of Total Proteins on Western Blots of 2-D Polyacrylamide Gels

    Detection of Total Proteins on Western Blots of 2-D Polyacrylamide Gels

    The ability of two-dimensional electrophoresis (2-DE), based on the original met

    2025-03-27 52
  • Target-Site Selection for the 1023 DNAzyme

    Target-Site Selection for the 1023 DNAzyme

    The 10–23 DNAzyme is capable of cleaving RNA with high sequence specificity at s

    2025-03-27 59
  • An efficient ligation method in the making of an in vitro virus for in vitro protein evolution

    An efficient ligation method in the making of an in vitro virus for in vitro protein evolution

    The “in vitro virus” is a molecular construct to perform evolutionary protein en

    2025-03-27 50
  • Application of a colorimetric assay to identify putative ribofuranosylaminobenzene 5-phosphate synth

    Application of a colorimetric assay to identify putative ribofuranosylaminobenzene 5-phosphate synth

    Tetrahydromethanopterin (H4 MPT) is a tetrahydrofolate analog originally discove

    2025-03-27 44
  • Analysis of Average Telomere Length in Cultured Human Cells

    Analysis of Average Telomere Length in Cultured Human Cells

    Telomeres play an important role in ensuring the integrity of the genome. Telome

    2025-03-27 56
  • Introduction to Telomeres and Telomerase

    Introduction to Telomeres and Telomerase

    Telomeres are ends of chromosomes that play an important part in the biology of

    2025-03-27 42
  • Probing the Telomere Damage Response

    Probing the Telomere Damage Response

    Telomere dysfunctions, rendered through replicative attrition of telomeric DNA o

    2025-03-27 48
  • Telomere Terminal G/C Strand Synthesis: Measuring Telomerase Action and C-Rich Fill-In

    Telomere Terminal G/C Strand Synthesis: Measuring Telomerase Action and C-Rich Fill-In

    Telomerase is present in most human cancers, and proliferative stem cells includ

    2025-03-27 54
  • Probing Antibody-Antigen Interactions by Mass Spectrometry

    Probing Antibody-Antigen Interactions by Mass Spectrometry

    Techniques that have been used for antigenic site mapping of linear epitopes in

    2025-03-27 38
  • Practical Considerations for Analyzing Antigene RNAs (agRNAs): RNA Immunoprecipitation of Argonaute

    Practical Considerations for Analyzing Antigene RNAs (agRNAs): RNA Immunoprecipitation of Argonaute

    Target validation for small RNAs in cells can be a confusing task wrought with p

    2025-03-27 45
  • Tandem Mass Spectrometry in the Detection of Inborn Errors of Metabolism for Newborn Screening

    Tandem Mass Spectrometry in the Detection of Inborn Errors of Metabolism for Newborn Screening

    Tandem mass spectrometry has been used for determinations of enzyme activities i

    2025-03-27 50