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Improved method for generating microarray probes using submicrogram amounts of total RNA
The ability to identify disease-associated genes using microarray technology is
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Peptide Nucleic Acid-Mediated Recombination for Targeted Genomic Repair and Modification
The ability to directly manipulate the human genome to correct a disease-related
2025-03-27 57 -
Site-Directed Mutagenesis
The ability to create defined mutations and to generate precise fusions of prote
2025-03-27 45 -
Restriction Endonuclease Digestion of DNA
The ability to cleave DNA at specific sites is one of the cornerstones of today’
2025-03-27 43 -
Detection of Total Proteins on Western Blots of 2-D Polyacrylamide Gels
The ability of two-dimensional electrophoresis (2-DE), based on the original met
2025-03-27 52 -
Target-Site Selection for the 1023 DNAzyme
The 10–23 DNAzyme is capable of cleaving RNA with high sequence specificity at s
2025-03-27 59 -
An efficient ligation method in the making of an in vitro virus for in vitro protein evolution
The “in vitro virus” is a molecular construct to perform evolutionary protein en
2025-03-27 50 -
Application of a colorimetric assay to identify putative ribofuranosylaminobenzene 5-phosphate synth
Tetrahydromethanopterin (H4 MPT) is a tetrahydrofolate analog originally discove
2025-03-27 44 -
Analysis of Average Telomere Length in Cultured Human Cells
Telomeres play an important role in ensuring the integrity of the genome. Telome
2025-03-27 56 -
Introduction to Telomeres and Telomerase
Telomeres are ends of chromosomes that play an important part in the biology of
2025-03-27 42 -
Probing the Telomere Damage Response
Telomere dysfunctions, rendered through replicative attrition of telomeric DNA o
2025-03-27 48 -
Telomere Terminal G/C Strand Synthesis: Measuring Telomerase Action and C-Rich Fill-In
Telomerase is present in most human cancers, and proliferative stem cells includ
2025-03-27 54 -
Probing Antibody-Antigen Interactions by Mass Spectrometry
Techniques that have been used for antigenic site mapping of linear epitopes in
2025-03-27 38 -
Practical Considerations for Analyzing Antigene RNAs (agRNAs): RNA Immunoprecipitation of Argonaute
Target validation for small RNAs in cells can be a confusing task wrought with p
2025-03-27 45 -
Tandem Mass Spectrometry in the Detection of Inborn Errors of Metabolism for Newborn Screening
Tandem mass spectrometry has been used for determinations of enzyme activities i
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