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  • CHO细胞表达系统在疫苗研制中的应用

    CHO细胞表达系统在疫苗研制中的应用

    分子生物学、分子免疫学等学科的发展使基因工程疫苗具有越来越重要的地位。在基因工程疫苗研究的动物细胞表达系统中,最具代表性的就是中国仓鼠卵巢细胞(Chinese

    2025-03-20 57
  • Southern Blotting of Genomic DNA from Lung and Its Tumors: Application to Analysis of Allele Loss on

    Southern Blotting of Genomic DNA from Lung and Its Tumors: Application to Analysis of Allele Loss on

    Lung cancer is one of the most common cancers afflicting the citizens of develop

    2025-03-20 47
  • RT-PCR Quantitation of HSP60 mRNA Expression

    RT-PCR Quantitation of HSP60 mRNA Expression

    Heat shock protein 60 (HSP60, HSPD1) is a “chaperonin” that facilitates folding

    2025-03-20 40
  • Selective Immortalization of Tumor-Specific T Cells to Establish Long-Term T-Cell Lines Maintaining

    Selective Immortalization of Tumor-Specific T Cells to Establish Long-Term T-Cell Lines Maintaining

    Antigen-specific T cells play a key role in cellular immune response against can

    2025-03-20 36
  • Phosphatidylinositol Kinase Activity in Ovarian Cancer Cells

    Phosphatidylinositol Kinase Activity in Ovarian Cancer Cells

    Phosphoinositides constitute less than 0.1% of total cellular lipids, yet accumu

    2025-03-20 28
  • 华人博士发明单个神经细胞及突触体内标识技术

    华人博士发明单个神经细胞及突触体内标识技术

    斯坦福大学生物学系神经生物学博士李凌,将在近期的《PLoS ONE》杂志上发表文章,宣布发明了一种对单个神经元细胞及突触进行体内标识的技术,并利用此技术成功地

    2025-03-20 28
  • Association of Nonreceptor Tyrosine Kinase c-Yes with Tight Junction Protein Occludin by Coimmunopre

    Association of Nonreceptor Tyrosine Kinase c-Yes with Tight Junction Protein Occludin by Coimmunopre

    Immunoprecipitation is one of the most commonly used techniques to study protein

    2025-03-20 36
  • FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas

    FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas

    Fluorescence In Situ Hybridization (FISH) is a powerful and robust technique all

    2025-03-20 27
  • Production of Ligand-Specific Mutants Using a Yeast Two-Hybrid Mating Assay

    Production of Ligand-Specific Mutants Using a Yeast Two-Hybrid Mating Assay

    Acquiring functional knowledge from protein-protein interaction studies often ne

    2025-03-20 27
  • Measurement of Multiple Drug Resistance Transporter Activity in Putative Cancer Stem/Progenitor Cell

    Measurement of Multiple Drug Resistance Transporter Activity in Putative Cancer Stem/Progenitor Cell

    Multiple drug resistance, mediated by the expression and activity of ABC-transpo

    2025-03-20 24
  • Simultaneous Humanization and Affinity Optimization of Monoclonal Antibodies

    Simultaneous Humanization and Affinity Optimization of Monoclonal Antibodies

    Monoclonal antibodies (MAbs) have broad therapeutic applications in cancer thera

    2025-03-20 30
  • Control of Mitotic Entry After DNA Damage in Drosophila

    Control of Mitotic Entry After DNA Damage in Drosophila

    In the presence of DNA damage, cells delay the entry into mitosis, presumably to

    2025-03-20 25
  • Apoptotic Caspase Activation and Activity

    Apoptotic Caspase Activation and Activity

    Caspases are central to the execution of apoptosis. Their proteolytic activity i

    2025-03-20 33
  • DNA-Mediated Gene Transfer

    DNA-Mediated Gene Transfer

    Transfection is the process by which exogenous DNA is transferred into eukaryoti

    2025-03-20 40
  • Analyzing the Spindle Checkpoint in Yeast and Frogs

    Analyzing the Spindle Checkpoint in Yeast and Frogs

    The spindle checkpoint is an evolutionarily conserved regulatory mechanism that

    2025-03-19 29