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A Computational Approach to Predict Suitable Target Sites for trans-Acting Minimal Hammerhead Ribozy
Trans-acting hammerhead ribozymes are challenging tools for diagnostic, therapeu
2025-03-31 53 -
Structural Determination of O-Glycans by Tandem Mass Spectrometry
Trans -sialidase (TS; E.C. 3.2.1.18) catalyzes the transfer of preferably α2,3-l
2025-03-31 41 -
Trans-Splicing Reactions by Ribozymes
Trans -cleaving ribozymes can be targeted to cut specific RNAs in vitro, which h
2025-03-31 56 -
Isotopic Efflux Studies as Indices of Phospholipase Activation
Traditionally, phospholipase activation is monitored in static incubations by th
2025-03-31 30 -
Noninvasive Bioluminescent Imaging of Infections
Traditional studies of viral and bacterial infection and pathogenesis have gener
2025-03-31 48 -
Fluorescence Approaches to Growing Macromolecule Crystals
Trace fluorescent labeling, typically less than 1%, can be a powerful aid in mac
2025-03-31 60 -
Extraction of Cellular and Tissue RNA
Total undegraded cellular RNA, largely free of contaminating DNA, can be rapidly
2025-03-31 43 -
EPR Measurements of Nitric Oxide-Induced Chromanoxyl Radicals of Vitamin E: Interactions with Vitami
Tocopherols (vitamin E) are the major lipid-soluble chain-breaking antioxidants
2025-03-31 47 -
Experimental Approaches for Measuring Reaction Parameters
To understand the function of a ribozyme, it is necessary to decipher the reacti
2025-03-31 57 -
Imaging Phosphoinositide Dynamics in Living Cells
To improve our understanding of the important roles played by inositol lipid der
2025-03-31 47 -
Automated Recombinant Protein Expression Screening in Escherichia coli
To fit the requirements of structural genomics programs, new as well as classica
2025-03-31 48 -
Cryopreservation of Hematopoietic Stem/Progenitor Cells for Therapeutic Use
To date, more than 25,000 hematopoietic transplants have been carried out across
2025-03-31 46 -
An in vitro method to select malignant cells from surgical biopsies of breast cancer patients
To date, breast cancer (BC) research is mainly studied with cell lines. These ce
2025-03-31 52 -
How to Perform a Nanoindentation Experiment on a Virus
To broaden our knowledge on virus structure and function, a profound insight int
2025-03-31 44 -
Inactivation of sortilin (a novel lysosomal sorting receptor) by dominant negative competition and R
To assess the role of sortilin in the sorting and trafficking of sphingolipid ac
2025-03-31 51
