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Preparation and Use of Tethered Ligands as Biomaterials and Tools for Cell Biology
The immobilization of biomolecules to various supports has been an important res
2025-03-29 74 -
Methods for Analysis of Unusual Forms of O-Glycosylation
The identification of the novel forms of O-linked glycosylation, O -fucose, and
2025-03-29 72 -
In Vitro Translation of mRNA in a Rabbit Reticulocyte Lysate Cell-Free System
The identification of specific messenger RNA molecules and the characterization
2025-03-29 83 -
Staining of Preparative 2-D Gels: Coomassie Blue and Imidazole-Zinc Negative Staining
The identification of proteins using preparative gel electrophoresis and mass sp
2025-03-29 77 -
Parkinsons Disease: Assays for the Ubiquitin Ligase Activity of Neural Parkin
The identification of monogenic variants of Parkinson’s disease (PD) has provide
2025-03-29 73 -
Nuclear Magnetic Resonance (NMR)-Based Drug Metabolite Profiling
The identification of drug metabolites in biofluids such as urine, plasma and bi
2025-03-29 83 -
Rapid identification of differentially expressed genes by in situ screening of bacteria
The identification of differentially expressed genes is a key step in the unders
2025-03-29 66 -
Antibody Epitope Mapping Using Arrays of Synthetic Peptides
The identification of antibody epitopes and their characterization at the amino
2025-03-29 60 -
Single-Strand Conformation Polymorphism (SSCP) Analysis
The identification of a large number of disease genes in recent years has led to
2025-03-29 87 -
High-resolution glycoprotein analysis using capillary electrophoresis
The high-resolution separation achievable with capillary electrophoresis has bee
2025-03-29 72 -
Procedures for the Analysis and Purification of His-Tagged Proteins
The heterologous expression of recombinant proteins is a valuable tool in the st
2025-03-29 63 -
Crystallization and Final Stages of Purification
The hard work has been done and the purification is “complete”—the target compou
2025-03-29 123 -
Enhancement of Ribozyme Function by RNA Binding Proteins
The hammerhead ribozyme motif can be engineered to function as a sequence-specif
2025-03-29 71 -
Minimized Hammerhead Ribozymes
The hammerhead ribozyme as engineered by Haseloff and Gerlach ( 1 ) consists of
2025-03-29 88 -
Design of the Hairpin Ribozyme for Targeting Specific RNA Sequences
The hairpin ribozyme has been developed and designed to target and cleave effici
2025-03-29 63