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Preparation, Restriction, and Hybridization Analysis of Mammalian Genomic DNA for Pulsed-Field Gel E
The aim of this chapter is to provide a very practical set of instructions that
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Purification and Enzymatic Sequencing of Polymerase Chain Reaction Products
The advent of direct sequencing of polymerase chain reaction (PCR) products has
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Generation of Epitope-Tagged GPCRs
The addition of one or more epitope tags to G-protein-coupled receptors (GPCRs)
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Acylcarnitines: Analysis in Plasma and Whole Blood Using Tandem Mass Spectrometry
The acylcarnitine profile is a diagnostic test for inherited disorders of fatty
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The Use of Translocating Fluorescent Biosensors for Real-Time Monitoring of GPCR-Mediated Signaling
The ability to visualize the subcellular localization of proteins by labeling th
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Systemic Delivery of Synthetic siRNAs
The ability to regulate endogenous gene expression by cleaving mRNA is important
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Spectrophotometric Analysis of Nucleic Acids
The ability to quantify nucleic acids accurately and rapidly is a prerequisite f
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Analysis of Urinary 8-oxo-7,8-dihydro-2-deoxyguanosine by Liquid ChromatographyTandem Mass Spectrome
The ability to non-invasively monitor DNA oxidation and its repair has significa
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Directed Mutagenesis of Antibody Variable Domains
The ability to mutate antibodies at the genetic level is a powerful tool in the
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Tobramycin Affinity Tag Purification of Spliceosomes
The ability to isolate native ribonucleoprotein (RNP) particles is of fundamenta
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Site-Directed Mutagenesis Using a Uracil-Containing Phagemid Template
The ability to introduce specific changes into almost any given DNA sequence has
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Improved method for generating microarray probes using submicrogram amounts of total RNA
The ability to identify disease-associated genes using microarray technology is
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Peptide Nucleic Acid-Mediated Recombination for Targeted Genomic Repair and Modification
The ability to directly manipulate the human genome to correct a disease-related
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Site-Directed Mutagenesis
The ability to create defined mutations and to generate precise fusions of prote
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Restriction Endonuclease Digestion of DNA
The ability to cleave DNA at specific sites is one of the cornerstones of today’
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