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Quantitation of Protein:Folin - phenol Reagent Method (Lowry Method)
【 Purpose 】 1.Study the principle and method of Lowry method to quantitate prote
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Western Blotting with Horseradish Pe
SAMPLE PREPARATIONFor Protein Concentration Determination of Cell Culture1.Decan
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Troubleshooting Guide: Western Blot
Use alternate buffer system (such as CAPS buffer pH 10.5) with a higher pH.Assem
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Western Blotting with Biotinylated Antibodies
SAMPLE PREPARATIONFor Protein Concentration Determination of Cell Culture1.Decan
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METHOD for Western Blots
1. While your SDS-PAGE gel is running, make your transfer buffer and chill to 4C
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Western Blotting with Monoclonal Ant
Sample PreparationFor Protein Concentration Determination of Cell Culture1.Decan
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Western Blot with BSA
Blotting with BSA1. Blot in 5% mile/ 1X PBS T (or 1X TBST) --60'/Rt2. Wash 2
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Postdoc Position in Biofuels Lab at Virginia Tech
Research Tools: Synthetic Biology and Protein EngineeringResearch Goals: (1)Impr
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Labeling Muscle Actin with 5-iodoacetamidofluorescein
Day 0 and 1Materials1.0.5 mM ATP,0.2 mM CaCl2,2 mM Tris-HCl,pH 8.0 at 4℃,250 ml
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Western Blotting with Alkaline Phosp
Western Blotting with Alkaline Phosphatase ConjugatesSAMPLE PREPARATIONFor Prote
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Filter Binding Assay for EBNA-1
Wash one 0.45um Millipore nitrocellulose filter (Cat# HAWP01300) in 1ml 0.4M KOH
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Protocol for anti-HA antibody Western Blotting
SJF 4/001) Run gel (in 1:10 running/transfer buffer(10x) and H2O for a total of
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ECL Western
(All steps with agitation, unless done O/N at 4℃)►Rinse membrane once in TBS-T►B
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Anti-P-CREB
1. Treat cells. For PC12 or NIH 3T3 cells a moderately confluent 60 mm plate is
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b-Galactosidase Activity Assay
Marian Price-Carter, 9/7/00Day 1 : Start overnight cultures in assay medium.Nega
