Isolation and Culture of Porcine Embryonic Stem Cells
Despite their agricultural and biomedical importance, embryonic stem cells (ESCs
Despite their agricultural and biomedical importance, embryonic stem cells (ESCs) are yet to be isolated for the pig or the domestic ungulates in general. This suggests that methods which have been used successfully in mice may not be applicable to these. In this chapter we describe a new method for the isolation of porcine ESCs. This method differs from those described previously in that it produces homogeneous outgrowths from undifferentiated inner cell mass cells when embryos are plated onto inactivated mouse embryonic feeder layers.
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Near-Infrared Optical Proteolytic Beacons for In Vivo Imaging of Matrix Metalloproteinase Activity
The exuberant expression of proteinases by tumor cells has l...
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Creation of Human Skin Equivalents for the In vitro Study of Angiogenesis in Wound Healing
In our efforts aimed at studying the cellular responses to i...