Assessment of Nanomaterials Cytotoxicity and Internalization
The impact that nanotechnology may have on life and medical sciences is immense and includes novel therapies as much as novel diagnostic and imaging tools, often offering the possibility to combine the two. It is, therefore, of the essence to understand and control the interactions that nanomaterials can have with cells, first at an individual level, focusing on, e.g., binding and internalization events, and then at a tissue level, where diffusion and long-range transport add further complications. Here, we present experimental methods based on selective labeling techniques and the use of effectors for a qualitative and quantitative evaluation of endocytic phenomena involving nanoparticles. The understanding of the cell–material interactions arising from these tests can then form the basis for a model-based evaluation of nanoparticles behavior in 3D tissues.
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Optimization of the Caco-2 Permeability Assay to Screen Drug Compounds for Intestinal Absorption and
In vitro permeability assays are a valuable tool for scienti...
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Quantitative Proteome and Phosphoproteome Analysis of Human Pluripotent Stem Cells
Understanding the signaling pathways governing pluripotency ...