Login
欢迎浏览恩派尔生物资料网
我要投稿 请登录 免费注册 安全退出

您现在的位置是: 首页 > 实验方法 > 细胞技术

细胞技术

Isolation and Culture of Bone Marrow-Derived Human Multipotent Stromal Cells (hMSCs)

2025-02-07 细胞技术 加入收藏
We have developed protocols whereby a total of 30–90 � 106 hMSCs with an average

We have developed protocols whereby a total of 30–90 � 106 hMSCs with an average viability greater than 90% can be produced in a single multilevel Cell Factory from a relatively small (1–3 mL) bone marrow aspirate in 14–20 d. It is possible to generate as many as 5 � 108 multipotent stromal cells (MSCs) from a single sample, depending on the number of Cell Factories seeded from the initial isolated hMSCs. Briefly, mononuclear cells are collected from a bone marrow aspirate by density gradient centrifugation. The cells are cultured overnight and the adherent cells are allowed to attach to the flask. Nonadherent cells are removed and the culture expanded for 7–10 d with periodic feeding of the cells. The cells are then harvested and seeded at low density (60–100 cells/cm2 ) into Nunc Cell Factories. The cells are allowed to expand for an additional 7–10 d, and are then harvested.

文章底部广告位

文章评论

加载中~