A Universal Nested Deletion Method Using an Arbitrary Primer and Elimination of a Unique Restriction

The generation of nested deletions within cloned fragments of DNA has important applications in molecular biology. For DNA sequencing, nested deletions provide a series of overlapping templates that can be used to generate a composite sequence with a single sequencing primer; in gene and protein functional studies, nested deletions allow researchers to locate and define a variety of functional domains based on regions of deletions.