Login
欢迎浏览恩派尔生物资料网
我要投稿 请登录 免费注册 安全退出

您现在的位置是: 首页 > 实验方法 > 细胞技术

细胞技术

Large-Scale Production and Storage of Monoclonal Antibodies and Hybridomas

2025-02-21 细胞技术 加入收藏
The end product of all the selection and cloning procedures described in Chapter

The end product of all the selection and cloning procedures described in Chapters 45 –48 will be a monoclonal hybridoma culture growing in a 0.2-mL culture well. From this single well, sufficient cells must be grown for storage in liquid nitrogen. It is advisable that at least six ampules, divided between two liquid nitrogen freezers, be stored. After the security of the cell line has been assured, then the clone can be further expanded for bulk antibody production. Large quantities of antibody may be produced either in ascitic fluid or in vitro in various culture vessels. The following sections describe the various areas covered in the chapter.

文章底部广告位

文章评论

加载中~