TISSUE CULTURE STOCK SOLUTIONS AND MEDIA

TISSUE CULTURE STOCK SOLUTIONS AND MEDIA MS MEDIUM FOR ARABIDOPSIS To 990 ml H2O add:Sucrose ........... 10.0 gMOPS .............. 0.5 gAgar .............. 8.0 gAdjust pH to 5.7 with 1 M KOH.Autoclave.When cooled add sterile stock solutions of:KNO3............... 20.0 ml 940 mM (95.00 g/L)NH4NO3............. 20.0 ml 1000 mM (80.04 g/L)KH2PO4............. 12.5 ml 100 mM (13.60 g/L)MgSO47H2O.......... 15.0 ml 100 mM (24.65 g/L)CaCl27H2O.......... 10.0 ml 300 mM (44.00 g/L)Glycine............ 2.67 ml 10 mMNicotinate......... 0.4 ml 10 mMThiamin............ 0.02 ml 10 mMPyridoxineHCl...... 0.24 ml 10 mMMyoinositol........ 5.5 ml 100 mMFeSO47H2O ......... 1.0 ml (2.780 g/100 ml)Na2EDTA ........... 1.0 ml 100 mM (3.720 g/100 ml)MnSO44H2O.......... 1.0 ml 100 mM (1.690 g/100 ml)ZnSO44H2O.......... 3.0 ml 10 mM (0.288 g/100 ml)H3BO3 ............. 1.0 ml 100 mM (0.618 g/100 ml)KI................. 0.5 ml 10 mM (0.166 g/100 ml)CuSO45H2O ......... 0.01 ml 10 mM (0.170 g/100 ml)Na2MoO42H2O ....... 0.1 ml 10 mM (0.242 g/100 ml)CoCl26H2O ......... 0.01 ml 10 mM (0.238 g/100 ml) B5 MEDIUM FOR ARABIDOPSIS To 990 ml H2O add:Sucrose ........... 20.0 gMOPS .............. 0.5 gAgar .............. 8.0 gAdjust pH to 5.7 with 1 M KOH.Autoclave.When cooled add sterile stock solutions of:KNO3............... 26.3 ml 940 mM (95.00 g/L)NaH2PO4H2O......... 11.0 ml 100 mMMgSO47H2O.......... 10.0 ml 100 mM (24.65 g/L)(NH4)2SO3.......... 10.0 ml 100 mMCaCl27H2O.......... 3.3 ml 300 mM (44.00 g/L)Myoinositol........ 5.5 ml 100 mMThiamine........... 3.0 ml 10 mMNicotinate......... 0.8 ml 10 mMPyridoxineHCl...... 0.48 ml 10 mM10X Micronutrients. 10.0 ml (see below)Sequestrene 330 Fe. 10.0 ml (0.28 g/100 ml)For Callus Inducing Medium (CIM) add:2,4-D..................... 0.5 mlKinetin................... 0.05 mlFor Shoot Inducing Medium (SIM) add:IAA....................... 0.15 ml6-dimethylaminopurine..... 5.0 ml 10X Micronutrients (per 100 ml)MnSO44H2O.......... 0.6 ml 100 mM (1.690 g/100 ml)ZnSO44H2O.......... 10.07 ml 10 mM (0.288 g/100 ml)H3BO3 ............. 4.9 ml 100 mM (0.618 g/100 ml)KI................. 4.5 ml 10 mM (0.166 g/100 ml)CuSO45H2O ......... 2.3 ml 10 mM (0.170 g/100 ml)Na2MoO42H2O ....... 1.0 ml 10 mM (0.242 g/100 ml)CoCl26H2O ......... 1.0 ml 10 mM (0.238 g/100 ml)