Login
欢迎浏览恩派尔生物资料网
我要投稿 请登录 免费注册 安全退出

您现在的位置是: 首页 > 实验方法 > 细胞技术

细胞技术

Activity Assays for Extracellular Signal-Regulated Kinase 5

2025-03-06 细胞技术 加入收藏
Extracellular signal-regulated kinase 5 (ERK5) is also known as big MAPK (BMK1)

Extracellular signal-regulated kinase 5 (ERK5) is also known as big MAPK (BMK1) or MAPK7. ERK5 is 115 kDa in mass and therefore larger than the other MAPKs such as ERK1/2, JNK, and p38. Like other MAPKs, ERK5 is ubiquitously expressed in mammalian cells and is part of a three kinase cascade involving a MAPK kinase (MEK5) and MAPK kinase kinase (primarily MEKK2 and MEKK3). ERK5 is important for proliferative responses to growth factors like epidermal growth factor and stress responses such as hyperosmolarity. Upon stimulation, ERK5 rapidly translocates to the nucleus for the control of transcription. ERK5 is also critical for maintenance of vascular integrity and endothelial cell survival. In this chapter, we define methods used to measure the activation of ERK5 using different biochemical and cell-based assays.

文章底部广告位

文章评论

加载中~