Login
欢迎浏览恩派尔生物资料网
我要投稿 请登录 免费注册 安全退出

您现在的位置是: 首页 > 实验方法 > 细胞技术

细胞技术

Keratinocyte Transient Transfections

2025-03-07 细胞技术 加入收藏
The goal of this chapter is to give the reader a concise and easy-to-follow guid

The goal of this chapter is to give the reader a concise and easy-to-follow guide to proven transient transfection techniques for primary strains and continuous lines of keratinocytes. The emphasis is on readily available and inexpensive resources that also allow for repeatability and adaptability to scale, experimental conditions, and sample replicates. In addition, basic cell culture techniques necessary to achieve optimal transfection results are provided. Although specified for keratinocytes, these techniques also work well for simple epithelial lines and common laboratory lines, such as COS-7 and CV-1. The standard precipitation protocol is suitable for many experimental designs, and the poly-L -ornithine protocol is intended for situations where calcium is contraindicated. The extended notes section provides necessary technical details to guide the novice user and reminders to the experienced scientist that success with the protocol will depend on several quantitative and qualitative factors including promoter strength, cell culture practices and plasmid isolation.

文章底部广告位

文章评论

加载中~