Login
欢迎浏览恩派尔生物资料网
我要投稿 请登录 免费注册 安全退出

您现在的位置是: 首页 > 实验方法 > 细胞技术

细胞技术

Tools to Investigate ROS Sensitive Signalling Proteins

2025-03-07 细胞技术 加入收藏
The thiols groups of cysteine residues on proteins are attractive oxidative targ

The thiols groups of cysteine residues on proteins are attractive oxidative targets for modification by reactive oxygen species, such as hydrogen peroxide (H2 O2 ). Such modification can lead to important cellular signaling processes that ultimately result in modification of physiology of the organism. To identify such proteins that are amenable to oxidative modification, different methods can be used. Here, two such approaches are described: one being the use of fluorescent thiol derivatives, and the second being the use of genetic mutants that are mutated in thiol residues. Using the model plant Arabidopsis thaliana , cell cultures, and whole plants, we describe these tools to help the reader understand the function of such thiol modification on plant responses.

文章底部广告位

文章评论

加载中~