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细胞技术

Isolation of DNA From Tumor Samples

2025-03-14 细胞技术 加入收藏
A method for producing high-molecular-weight DNA from pulverized tissue, nuclear

A method for producing high-molecular-weight DNA from pulverized tissue, nuclear fractions, or cultured cells. This isolation method relies on the powerful proteolytic activity of proteinase K combined with the denaturing ability of the ionic detergent sodium dodecyl sulfate. Ethylenediaminetetraacetic acid is included in the lysis buffer to inhibit DNases.

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