Login
欢迎浏览恩派尔生物资料网
我要投稿 请登录 免费注册 安全退出

您现在的位置是: 首页 > 实验方法 > 细胞技术

细胞技术

Flow Cytometric Measurement of Functional and Phenotypic P-Glycoprotein

2025-03-14 细胞技术 加入收藏
The measurement of functional and phenotypic P-glycoprotein by flow cytometry is

The measurement of functional and phenotypic P-glycoprotein by flow cytometry is suitable for cells in suspension, and is particularly appropriate for blood and bone marrow cells. We describe a functional assay for P-glycoprotein using rhodamine 123, an assay for daunorubicin accumulation, and an assay to measure P-glycoprotein levels using the MRK16 antibody. Our protocols include the use of an anti-CD45 antibody for the identification of leukemic blasts. The protocols described in this chapter were designed for use in studies accompanying UK Medical Research Council Trials of patients with Acute Myeloid Leukaemia and High Risk Myelodysplastic Syndrome (≫10‰ blasts). These assays are performed in more than one UK Centre, and hence each assay has been subjected to rigorous reproducibility testing (1 ).

文章底部广告位

文章评论

加载中~