Login
欢迎浏览恩派尔生物资料网
我要投稿 请登录 免费注册 安全退出

您现在的位置是: 首页 > 实验方法 > 细胞技术

细胞技术

Dideoxy DNA sequencing is routinely used in research and, increasingly, in clinical care for the det

2025-03-17 细胞技术 加入收藏
Western blotting is utilized to detect and quantify the amount of a specific pro

Western blotting is utilized to detect and quantify the amount of a specific protein in a complex mixture of proteins and at the same time to determine its molecular weight. In particular, by Western blotting it is also possible to detect and discriminate the two isoforms of nm23, H1, and H2 (1, 2). nm23-H1 is about 18.5 kD (3 ) and nm23-H2 is 17 kD (4 ), hence, they migrate as two distinct bands on a standard SDS polyacrylamide gel. Because the two isoforms are very highly conserved (4 ), they retain most of the same epitopes; thus any polyclonal antibody raised against one of the two isoforms or a monoclonal antibody against a conserved epitope will recognize both nm23-H1 and -H2 at the same time. Analysis by immunohistochemistry, on the other hand would require two distinct reactions with two specific antibodies for H1 and H2 raised against the least conserved epitopes to avoid crossreaction.

文章底部广告位

文章评论

加载中~