Login
欢迎浏览恩派尔生物资料网
我要投稿 请登录 免费注册 安全退出

您现在的位置是: 首页 > 实验方法 > 细胞技术

细胞技术

TNF Signaling: Key Protocols

2025-03-19 细胞技术 加入收藏
Tumor necrosis factor (TNF) is a pleiotropic cytokine that signals inflammation

Tumor necrosis factor (TNF) is a pleiotropic cytokine that signals inflammation as well as cell death. We focus herein on the inflammatory pathway, giving particular emphasis to the in vitro methods used to study intracellular signaling mediators. The signal transduction cascade that TNF triggers after binding to the TNF receptors (TNFRs), flows throughout a series of protein-protein interactions as well as kinase activations, and finally leads to the translocation of distinct transcription factors within the nucleus, eventually inducing the transcription of specific inflammatory genes. In this chapter, we describe the analysis of the TNF receptor signaling complex by immunoprecipitation (IP), the activation of different MAP kinases by Western blot, the analysis of transcription factor activation by either electrophoretic mobilityshift assay (EMSA) or reporter assay, and the analysis of TNF-induced genes by chromatin IP (ChIP).

文章底部广告位

文章评论

加载中~