Login
欢迎浏览恩派尔生物资料网
我要投稿 请登录 免费注册 安全退出

您现在的位置是: 首页 > 实验方法 > 细胞技术

细胞技术

Ultrasound-Guided Intracardial Injection and In Vivo Magnetic Resonance Imaging of Single Cells in M

2025-03-19 细胞技术 加入收藏
Magnetic resonance imaging (MRI) has become an important technique for noninvasi

Magnetic resonance imaging (MRI) has become an important technique for noninvasive cell tracking in preclinical research. Following appropriate cell labeling MRI can be used to detect larger cell cohorts and also single cells in vivo in mice. Cell distribution to different organs such as brain, liver, spleen, and kidneys can be visualized, semi-quantified, and followed over time. Thus, the fate of single tumor cells and their eventual development to solid metastases could be investigated. Mesenchymal stromal cells can be used as a paradigm for metastasizing tumor cells. We have demonstrated a strategy for magnetic and fluorescent co-labeling of mesenchymal stromal cells (MSC), ultrasound-guided intracardial cell injection with efficient systemic cell delivery, and high-resolution MRI for repetitive visualization of disseminated co-labeled MSC on a single-cell level in vivo in mice. Furthermore, the fluorescent labeling of cells enabled effective histopathological validation.

文章底部广告位

文章评论

加载中~