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High-Throughput Analysis of Serum Antigens Using Sandwich ELISAs on Microarrays

2025-03-19 细胞技术 加入收藏
Enzyme-linked immunosorbent assay (ELISA) microarrays promise to be a powerful t

Enzyme-linked immunosorbent assay (ELISA) microarrays promise to be a powerful tool for the detection and validation of disease biomarkers. ELISA microarrays are capable of simultaneous detection of many proteins using a small sample volume. Although there are many potential pitfalls to the use of ELISA microarrays, these can be avoided by careful planning of experiments. In this chapter we describe a high-throughput protocol for processing ELISA microarrays that will result in reliable and reproducible data.

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