RT-PCR Quantitation of HSP60 mRNA Expression

Heat shock protein 60 (HSP60, HSPD1) is a “chaperonin” that facilitates folding of nascent proteins into proper conformations (1 ). It is thought to play a critical role in the assembly, folding, and transport of proteins in the mitochondria. HSP60 also interacts with nascent cellular proteins to prevent their denaturation under heat stress (2 ). The HSP60 gene sequence is known and is highly conserved (3 ). Expression of the HSP60 gene has been associated with cisplatin resistance in several preclinical model systems and in ovarian carcinoma patients (1 -6 ); we quantitated HSP60 mRNA expression in preclinical human ovarian and bladder carcinoma models .